High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human Monocytes In Vitro
Autor: | Galileo Escobedo, Guillermo Melendez-Mier, Angélica Y. Gómez-Arauz, Felix Rückert, Sergio Islas-Andrade, José Manuel Fragoso, Johanna L. Grün, Aarón N. Manjarrez-Reyna, Nallely Bueno-Hernández, Sonia Leon-Cabrera |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Adult Lipopolysaccharides Male lcsh:Immunologic diseases. Allergy medicine.medical_specialty Article Subject Lipopolysaccharide Diet therapy Immunology Interleukin-1beta Primary Cell Culture Monocytes Flow cytometry 03 medical and health sciences chemistry.chemical_compound Young Adult High-density lipoprotein Internal medicine medicine Immunology and Allergy Humans Cells Cultured Metabolic Syndrome medicine.diagnostic_test Chemistry Monocyte Interleukin General Medicine Middle Aged medicine.disease Flow Cytometry 030104 developmental biology medicine.anatomical_structure Endocrinology Female lipids (amino acids peptides and proteins) Metabolic syndrome Lipoproteins HDL lcsh:RC581-607 Lipoprotein Diet Therapy Research Article |
Zdroj: | Journal of Immunology Research, Vol 2018 (2018) Journal of Immunology Research |
ISSN: | 2314-7156 2314-8861 |
Popis: | The effect of metabolic syndrome on human monocyte subpopulations has not yet been studied. Our main goal was to examine monocyte subpopulations in metabolic syndrome patients, while also identifying the risk factors that could directly influence these cells. Eighty-six subjects were divided into metabolic syndrome patients and controls. Monocyte subpopulations were quantified by flow cytometry, and interleukin- (IL-) 1β secretion levels were measured by ELISA. Primary human monocytes were cultured in low or elevated concentrations of high-density lipoprotein (HDL) and stimulated with lipopolysaccharide (LPS). The nonclassical monocyte (NCM) percentage was significantly increased in metabolic syndrome patients as compared to controls, whereas classical monocytes (CM) were reduced. Among all metabolic syndrome risk factors, HDL reduction exhibited the most important correlation with monocyte subpopulations and then was studied in vitro. Low HDL concentration reduced the CM percentage, whereas it increased the NCM percentage and IL-1β secretion in LPS-treated monocytes. The LPS effect was abolished when monocytes were cultured in elevated HDL concentrations. Concurring with in vitro results, IL-1β serum values significantly increased in metabolic syndrome patients with low HDL levels as compared to metabolic syndrome patients without HDL reduction. Our data demonstrate that HDL directly modulates monocyte subpopulations in metabolic syndrome. |
Databáze: | OpenAIRE |
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