Murine gamma interferon activates the release of a macrophage-derived Ia-inducing factor that transfers Ia inductive capacity
| Autor: | Noel L. Warner, V Maino, M Sanchez-Lanier, E B Walker, C Stewart |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
medicine.drug_class
Immunology Cell Lymphocyte Cooperation Biology Monoclonal antibody Antiviral Agents Binding Competitive Antibodies Chromatography Affinity Cell Line Interferon-gamma Mice Antigen medicine Concanavalin A Immunology and Allergy Animals Interferon gamma Lymphokines Monocyte Macrophages Histocompatibility Antigens Class II Biological activity Articles Macrophage Activation Chromatography Agarose Flow Cytometry Molecular biology Rats medicine.anatomical_structure Cell culture Macrophage-Activating Factors Antigens Surface biology.protein medicine.drug |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | In this report we demonstrate that when the murine macrophage tumor cell line P- 388D1 is incubated for 48-72 h with either concanavalin A-stimulated rat spleen cell supernatant or cloned murine immune interferon (IFN-gamma), the cultured cells release a cell-free factor activity that in turn induces the cell surface expression of Ia antigen on the murine monocyte cell line WEHI-3. This IFN-gamma-stimulated, Ia-inducing activity cannot be blocked with an anti-IFN-gamma heteroantiserum that does block the induction of Ia expression on WEHI-3 by both cloned murine IFN-gamma and rat Con A supernatant. The Ia-inducing factor ( IaIF ) generated from P- 388D1 after stimulation by IFN-gamma does not demonstrate any antiviral activity. The P- 388D1 -derived IaIF is not shed plasma membrane Ia glycoprotein molecules, as demonstrated by the inability of the active component to bind specifically to an anti-I-Ad affinity column or to a protein A column after the active supernatant is first treated with an excess of anti-I-E/Cd,k monoclonal antibody. |
| Databáze: | OpenAIRE |
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