Enzymolysis–Microwave-Assisted Hydrodistillation for Extraction of Volatile Oil from Atractylodes Chinensis and Its Hypoglycemic Activity in vitro
| Autor: | Ruiqing Qin, Meixing Yan, Yanling Gong, Yitong Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Residue (complex analysis) Chromatography 010405 organic chemistry Chemistry Family Asteraceae 010401 analytical chemistry Extraction (chemistry) Microwave power Atractylodes 01 natural sciences Microwave assisted Gas Chromatography-Mass Spectrometry 0104 chemical sciences Analytical Chemistry Atractylodes chinensis Oils Volatile Hypoglycemic Agents Environmental Chemistry Response surface methodology Gas chromatography–mass spectrometry Microwaves Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 104:1196-1205 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoacint/qsab008 |
| Popis: | Background Atractylodes chinensis (family Asteraceae) is a perennial herb with many pharmacological effects. Objective Extraction of volatile oil from Atractylodes chinensis was optimized and its hypoglycemic activities were studied. Methods Enzymolysis–microwave-assisted hydrodistillation (EMAHD) was adopted to extract the volatile oil, and the technology was optimized using a single-factor experiment that incorporated response surface methodology (RSM). The extraction rates of volatile oil by EMAHD, microwave-assisted hydrodistillation (MAHD), and hydrodistillation (HD) methods were compared at different times. The ingredients of Atractylodes chinensis volatile oil were analyzed by gas chromatography–mass spectrometry. Scanning electron microscopy (SEM) were used to analyze the microstructural changes in Atractylodes chinensis residue before and after extraction. The inhibition of α-amylase activity was determined. Results The obtained optimal extraction conditions for EMAHD were as follows: enzyme concentration 1.6%, pH 7, enzymolysis time 20 min, enzymolysis temperature 50°C, liquid–solid ratio 30:1, microwave power 455 W, and microwave time 40 min. The levels of the main ingredients and the in vitro inhibition of α-amylase activities were higher for Atractylodes chinensis volatile oil extracted by EMAHD than by HD or MAHD. The powder residue of Atractylodes chinensis remaining after EMAHD showed a ruptured and collapsed cell structure, indicating accelerated dissolution of the volatile oil. Conclusions and Highlights EMAHD is deemed a method with many advantages for extraction of volatile oil from Atractylodes chinensis. The volatile oil of Atractylodes chinensis is a promising component for treating hyperglycemia. |
| Databáze: | OpenAIRE |
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