Pulmonary inflammation monitored noninvasively by MRI in freely breathing rats
| Autor: | Nicolau Beckmann, Bruno Tigani, Elisabeth Schaeublin, Alan D. Jackson, Rosemary Sugar, John R. Fozard |
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| Rok vydání: | 2002 |
| Předmět: |
Budesonide
Lipopolysaccharides Male Eosinophil Peroxidase Ovalbumin Biophysics Anti-Inflammatory Agents Inflammation Pulmonary Edema Biochemistry Pulmonary Disease Chronic Obstructive In vivo Edema Rats Inbred BN medicine Animals Pulmonary Eosinophilia Molecular Biology Lung Asthma business.industry Respiration Cell Biology respiratory system Eosinophil Allergens medicine.disease Mucus Magnetic Resonance Imaging respiratory tract diseases Rats medicine.anatomical_structure Peroxidases Immunology medicine.symptom business Bronchoalveolar Lavage Fluid medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 292(1) |
| ISSN: | 0006-291X |
| Popis: | A detailed analysis has been carried out of the correlation between the signals detected by MRI in the rat lung after allergen or endotoxin challenge and parameters of inflammation determined in the broncho-alveolar lavage (BAL) fluid. MRI signals after allergen correlated highly significantly with the BAL fluid eosinophil number, eosinophil peroxidase activity and protein concentration. Similar highly significant correlations were seen when the anti-inflammatory glucocorticosteroid, budesonide, manifested against allergen. In contrast, following endotoxin challenge, mucus was the sole BAL fluid parameter that correlated significantly with the long lasting signal detected by MRI. Since edema is an integral component of pulmonary inflammation, MRI provides a noninvasive means of monitoring the course of the inflammatory response and should prove invaluable in profiling anti-inflammatory drugs in vivo. Further, the prospect of noninvasively detecting a sustained mucus hypersecretory phenotype in the lung brings an important new perspective to models of chronic obstructive pulmonary diseases. |
| Databáze: | OpenAIRE |
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