Proteomic profiling in musculoskeletal oncology by MALDI mass spectrometry
| Autor: | Robert L. Caldwell, Herbert S. Schwartz, Ginger E. Holt |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Maldi ms Protein Array Analysis Cell Cycle Proteins Soft Tissue Neoplasms Computational biology Proteomics Mass spectrometry S100 Calcium Binding Protein A6 medicine Carcinoma Humans Orthopedics and Sports Medicine Proteomic Profiling business.industry Soft tissue sarcoma S100 Proteins Sarcoma General Medicine medicine.disease Immunohistochemistry Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Surgery business Glioblastoma |
| Zdroj: | Clinical orthopaedics and related research. 450 |
| ISSN: | 0009-921X |
| Popis: | UNLABELLED Proteomics is the emerging technology that evaluates normal and abnormal protein expression in tissues. Tissue profiling by matrix assisted laser desorption ionization mass spectrometry (MALDI MS) is a proteomic tool that permits the rapid detection of proteins expressed in tissues and serum. To date, tissue profiling has been successfully utilized to detect proteins specific to various cancers, including those of non-small cell lung carcinoma and glioblastoma multiforme. The usefulness of histological analyses to predict soft tissue sarcoma (STS) behavior has plateaued, and a critical need exists to identify other means to objectively predict tumor grade and behavior. We describe tissue profiling as a tool for orthopaedic research, and review recent work utilizing the technology for detecting proteins that differentiate low grade from high grade STS. We used MALDI MS tissue profiling technology to detect differentially expressed proteins in high grade and low grade STS. LEVEL OF EVIDENCE Level I, prognostic study. |
| Databáze: | OpenAIRE |
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