Vascular effects of advanced glycation end-products : content of immunohistochemically detected AGEs in radial artery samples as a predictor for arterial calcification and cardiovascular risk in asymptomatic patients with chronic kidney disease
| Autor: | Krzanowska, Katarzyna, Krzanowski, Marcin, Gajda, Mariusz, Dumnicka, Paulina, Jasek-Gajda, Ewa, Fedak, Danuta, Pietrzycka, Agata, Kuźniewski, Marek, Litwin, Jan, Sułowicz, Władysław |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Glycation End Products
Advanced Male medicine.medical_specialty Article Subject alpha-2-HS-Glycoprotein Clinical Biochemistry Asymptomatic Gastroenterology Picrates Calcinosis Diabetes mellitus Internal medicine Plasminogen Activator Inhibitor 1 Genetics medicine Humans Renal Insufficiency Chronic Molecular Biology Aged Medial arterial calcification lcsh:R5-920 biology business.industry Biochemistry (medical) C-reactive protein Biphenyl Compounds General Medicine Middle Aged medicine.disease Biphenyl compound Arterial calcification Endocrinology C-Reactive Protein Cardiovascular Diseases Radial Artery biology.protein Female medicine.symptom lcsh:Medicine (General) business Biomarkers Kidney disease Research Article |
| Zdroj: | Disease Markers Disease Markers, Vol 2015 (2015) |
| Popis: | Objectives.Our aim was to determine whether vascular deposition of advanced glycation end-products (AGEs) is associated with arterial calcification and cardiovascular mortality in chronic kidney disease (CKD) patients and to assess the relationships between vascular content of AGEs and selected clinical and biochemical parameters.Materials and Methods.The study comprised 54 CKD patients (33 hemodialyzed, 21 predialyzed). Examined parameters included BMI, incidence of diabetes, plasma fasting glucose, AGEs, soluble receptor for AGEs and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, serum C-reactive protein (hsCRP), plasminogen activator inhibitor-1 (PAI-1), and fetuin-A. Fragments of radial artery obtained during creation of hemodialysis access were stained for calcifications using alizarin red. AGEs deposits were identified immunohistochemically and their relative content was quantified.Results.Vascular content of AGEs was positively correlated with BMI, hsCRP, fetuin-A, PAI-1, and DPPH scavenging in simple regression; only fetuin-A was an independent predictor in multiple regression. There was a significant positive trend in the intensity of AGEs immunostaining among patients with grades 1, 2, and 3 calcifications. AGEs immunostaining intensity predicted 3-year cardiovascular mortality irrespective of patient’s age.Conclusions.The present study demonstrates an involvement of AGEs in the development of medial arterial calcification and the impact of arterial AGE deposition on cardiovascular mortality in CKD patients. |
| Databáze: | OpenAIRE |
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