Genetic linkage between serogroup specificity and antibiotic resistance in Neisseria gonorrhoeae
| Autor: | Marianne Bäckman, Dan Danielsson, Bygdeman S, Mari Norgren |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.drug_class
Genetic Linkage Penicillin Resistance Antibiotics Immunoelectrophoresis Penicillins Biology medicine.disease_cause Microbiology Antibiotic resistance Transformation Genetic medicine Serotyping General Immunology and Microbiology medicine.diagnostic_test Strain (chemistry) General Medicine Neisseria gonorrhoeae Anti-Bacterial Agents Penicillin Transformation (genetics) Genes Bacterial Bacterial outer membrane medicine.drug |
| Zdroj: | Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology. 90(3) |
| ISSN: | 0108-0180 |
| Popis: | In previous works statistically significant differences were demonstrated in antibiotic susceptibility between gonococcal strains of the recently described W serogroups, W I, W II and W III, respectively. Strains of serogroup W I were almost always sensitive to penicillin and other antibiotics while those of the W II and W III serogroups showed a higher incidence of decreased susceptibility. Transformation experiments were therefore undertaken with an antibiotic sensitive serogroup W I gonococcal strain as recipient and a multi-resistant W II strain as DNA-donor. Transformants, with increased resistance to penicillin and several other antibiotics as compared with the recipient, acquired the same serogroup specificity as the W II donor. With one of these W II transformants as donor and the sensitive W I strain as recipient all transformants acquired the same antibiotic susceptibility pattern as well as the same serogroup as the donor. SDS-PAGE, performed on sarkosyl extracted outer membrane proteins from donor, recipient and some transformants, showed an increase in the molecular weight of the Protein I of the outer membrane of the W II transformants as compared with that of the recipient strain. In rocket-line and crossed-line immunoelectrophoresis the W II transformants could not be distinguished from the W II donor strain. A genetic linkage between antibiotic multi-resistance and serogroup W II specificity was thus shown. This is in agreement with the demonstrated higher incidence of W II strains with increased antibiotic resistance as compared with that of serogroup W I strains. |
| Databáze: | OpenAIRE |
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