Endogenously produced glycosaminoglycans affecting the release of lipoprotein lipase from macrophages and the interaction with lipoproteins
| Autor: | Rudolf Winkler, Robert Zimmermann, Eva Hurt-Camejo, Peter Sartipy, Rudolf Zechner, Gert M. Kostner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Arteriosclerosis
Lipoproteins Dermatan Sulfate Cell Line Glycosaminoglycan chemistry.chemical_compound Humans Macrophage Chondroitin sulfate Molecular Biology Glycosaminoglycans Foam cell Lipoprotein lipase Heparin Macrophages Chondroitin Sulfates Cell Biology Lipoproteins LDL Lipoprotein Lipase Biochemistry chemistry Cell culture Culture Media Conditioned Low-density lipoprotein Phorbol Proteoglycans lipids (amino acids peptides and proteins) |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1484:316-324 |
| ISSN: | 1388-1981 |
| Popis: | Macrophages are intimately involved in the pathogenesis of atherosclerotic diseases. A key feature of this process is their uptake of various lipoproteins and subsequent transformation to foam cells. Since lipoprotein lipase (LPL) is believed to play a role in foam cell formation, we investigated if endogenously produced proteoglycans (PGs) affect the release of this enzyme from macrophages. The human leukaemic cell line THP-1 which differentiates into macrophages by treatment with phorbol ester (phorbol 12-myristate 13-acetate) served as a model. The differentiation of THP-1 macrophages promoted the release of PGs into the cell medium which caused the detachment of LPL activity from the cell surface, and prevented LPL re-uptake and inactivation. These PGs were mainly composed of chondroitin sulfate type and exerted a heparin-like effect on LPL release. LPL is known to increase the cell association of lipoproteins by the well known bridging function. Exogenous bovine LPL at a concentration of 1 μg/ml enhanced low density lipoprotein (LDL)-binding 10-fold. Endogenously produced PGs reduced LPL-mediated binding of LDL. It is proposed that the differentiation-dependent increase in the release of PGs interferes with binding of LPL and reduces lipoprotein-binding to macrophages. |
| Databáze: | OpenAIRE |
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