Nitric oxide inhibits the ATPase activity of the chaperone-like AAA+ ATPase CDC48, a target for S-nitrosylation in cryptogein signalling in tobacco cells
| Autor: | Stéphane Bourque, David Wendehenne, Jean Borges Bertoldo, Hernán Terenzi, Jeremy Astier, Angélique Besson-Bard, Olivier Lamotte |
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| Přispěvatelé: | Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Federal de Santa Catarina = Federal University of Santa Catarina [Florianópolis] (UFSC), Agence Nationale de la Recherche [BLAN07-2_184783 PIANO], Burgundy State [PARI AGRALE 8], FAPESC (Fundacao de Apoio a Pesquisa Cientifica e Tecnologica do Estado de Santa Catarina), CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), MCT/FINEP (Ministerio da Ciencia e Tecnologia/Financiadora de Estudos e Projetos), CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior), Ministere Francais de l'Education Nationale, de la Recherche et de la Technologie, Burgundy Sate [FABER project] [PARI AGRALE 8] |
| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular 0106 biological sciences Protein Conformation [SDV]Life Sciences [q-bio] Nicotiana tabacum ATPase Molecular Sequence Data Cell Cycle Proteins Nitric Oxide 01 natural sciences Biochemistry cryptogein Fungal Proteins 03 medical and health sciences Valosin Containing Protein Tobacco Amino Acid Sequence nitric oxide (no) Molecular Biology Plant Proteins 030304 developmental biology Adenosine Triphosphatases 0303 health sciences biology Walker motifs Cell Biology S-Nitrosylation cell division cycle 48 (cdc48) Biotic stress biology.organism_classification AAA proteins Protein Structure Tertiary Elicitor Chaperone (protein) [SDE]Environmental Sciences biology.protein s-nitrosylation plant defence responses 010606 plant biology & botany |
| Zdroj: | Biochemical Journal Biochemical Journal, Portland Press, 2012, 447, pp.249-260. ⟨10.1042/BJ20120257⟩ |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | NO has important physiological functions in plants, including the adaptative response to pathogen attack. We previously demonstrated that cryptogein, an elicitor of defence reaction produced by the oomycete Phytophthora cryptogea , triggers NO synthesis in tobacco. To decipher the role of NO in tobacco cells elicited by cryptogein, in the present study we performed a proteomic approach in order to identify proteins undergoing S-nitrosylation. We provided evidence that cryptogein induced the S-nitrosylation of several proteins and identified 11 candidates, including CDC48 (cell division cycle 48), a member of the AAA+ ATPase (ATPase associated with various cellular activities) family. In vitro , NtCDC48 ( Nicotiana tabacum CDC48) was shown to be poly-S-nitrosylated by NO donors and we could identify Cys 110 , Cys 526 and Cys 664 as a targets for S-nitrosylation. Cys 526 is located in the Walker A motif of the D2 domain, that is involved in ATP binding and was previously reported to be regulated by oxidative modification in Drosophila . We investigated the consequence of NtCDC48 S-nitrosylation and found that NO abolished NtCDC48 ATPase activity and induced slight conformation changes in the vicinity of Cys 526 . Similarly, substitution of Cys 526 by an alanine residue had an impact on NtCDC48 activity. More generally, the present study identified CDC48 as a new candidate for S-nitrosylation in plants facing biotic stress and further supports the importance of Cys 526 in the regulation of CDC48 by oxidative/nitrosative agents. |
| Databáze: | OpenAIRE |
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