Hyposmolar medium and ethanol in isosmotic solution induce the release of thyrotropin-releasing hormone (TRH) by isolated rat pancreatic islets
| Autor: | Monte A. Greer, Július Benický, Vladimír Štrbák |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Thyrotropin-releasing hormone Stimulation General Biochemistry Genetics and Molecular Biology Islets of Langerhans chemistry.chemical_compound Internal medicine medicine Animals Rats Wistar General Pharmacology Toxicology and Pharmaceutics Thyrotropin-Releasing Hormone Incubation Cells Cultured Cell Size geography Ethanol geography.geographical_feature_category Cell swelling Pancreatic islets Central Nervous System Depressants General Medicine Islet Stimulation Chemical Rats Kinetics Endocrinology medicine.anatomical_structure Hypotonic Solutions chemistry Tonicity Calcium Isotonic Solutions |
| Zdroj: | Life Sciences. 60:865-872 |
| ISSN: | 0024-3205 |
| Popis: | Cell swelling induced by hypotonic medium or small isotonic permeant molecules results in an immediate secretory response in various types of cells. We have expanded exploration of this phenomenon by examining the effect of either isotonic ethanol or hyposmotic medium on the release of TRH by freshly isolated islets of Langerhans in static incubation and perifusion. Ethanol (40, 80 or 160 mM in isotonic solution) dose-dependently evoked the release of TRH by statically incubated islets. The dynamics of TRH release induced by 80 mM isotonic ethanol or 30% hypotonic medium were similar to those induced by 50 mM KCl, with the highest secretion rate during the first 5 min of incubation irrespective of the duration of stimulation. Ca2+ depletion of the incubation medium abolished the response to 50 mM KCl but did not diminish the response to 80 mM isotonic ethanol. We conclude that osmotic stimuli known to induce cell swelling also induce release of TRH by isolated pancreatic islets. |
| Databáze: | OpenAIRE |
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