mir124-dependent tagging of glutamatergic synapses by synaptopodin controls non-uniform and input-specific homeostatic synaptic plasticity
Autor: | Alexandre Favereaux, Olivier Thoumine, Béatrice Tessier, Sébastien Benquet, Mathieu Letellier, Sandra Dubes, Anaïs Soula, Christel Poujol |
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Přispěvatelé: | Interdisciplinary Institute for Neuroscience [Bordeaux] (IINS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2021 |
Předmět: |
0303 health sciences
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology AMPA receptor Biology Spine apparatus 03 medical and health sciences Glutamatergic 0302 clinical medicine Hebbian theory medicine.anatomical_structure Synaptic plasticity medicine Premovement neuronal activity Synaptopodin Neuron Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
Popis: | SummaryHomeostatic synaptic plasticity (HSP) is a process by which neurons adjust synaptic strengths to compensate for various perturbations and which allows to stabilize neuronal activity. Yet, whether the highly diverse synapses harboring a neuron respond uniformly to a same perturbation is unclear and the underlying molecular determinants remain to be identified. Here, using patch-clamp recordings, immunolabeling and imaging approaches, we report that the ability of individual synapses to undergo HSP in response to activity-deprivation paradigms depends on the local expression of the spine apparatus related protein synaptopodin (SP) acting as a synaptic tag to promote AMPA receptor synaptic accumulation and spine growth. Gain and loss-of-function experiments indicate that this process relies on the local de-repression of SP translation by miR124 which supports both non-uniform and synapse-autonomous HSP induced by global or inputspecific activity deprivation, respectively. Our findings uncover an unexpected synaptic-tagging mechanism for HSP, whose molecular actors are intriguingly shared with Hebbian plasticity and linked to multiple neurological diseases. |
Databáze: | OpenAIRE |
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