Derivatives form better lipoxygenase inhibitors than piperine: in vitro and in silico study
| Autor: | M. J. Tomy, Chittalakkottu Sadasivan, Abudulhameed Sabu, Madathilkovilakathu Haridas, Chelankara Suresh Sharanya, Shankar Prasanth, K. V. Dileep |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Stereochemistry Polyunsaturated Alkamides Lipoxygenase Secondary metabolite Biochemistry Benzoates chemistry.chemical_compound Alkaloids Piperidines Catalytic Domain Drug Discovery medicine Organic chemistry Humans Computer Simulation Enzyme kinetics Benzodioxoles Lipoxygenase Inhibitors Pharmacology biology Organic Chemistry Active site Piperonal chemistry Piperine Benzaldehydes biology.protein Fatty Acids Unsaturated Molecular Medicine Fermentation Soybeans Piper nigrum Piperic acid medicine.drug Protein Binding |
| Zdroj: | Chemical biologydrug design. 85(6) |
| ISSN: | 1747-0285 |
| Popis: | Piperine is a secondary metabolite of black pepper. Its uses in medicine were already studied. However, its derivatives have not gained considerable attention. In the presented study, the Lipoxygenase (LOX) inhibitory activity of piperine and its derivatives, piperonylic acid, piperic acid, and piperonal have been assessed and compared by enzyme kinetics, ITC and molecular modeling experiments. The presented investigations expressed that all the studied compounds inhibited LOX by binding at its active site. The IC50 values of these compounds were deduced from the kinetics data and found to be 85.79, 43.065, 45.17, and 50.78 lM for piperine, piperonylic acid, piperic acid, and piperonal, respectively. The binding free energies obtained from ITC experiments were � 7.47, � 8.33, � 8.09, and � 7.86 kcal/mol for piperine, piperonylic acid, piperic acid, and piperonal, respectively. Similarly, the glide scores obtained for piperine, piperonylic acid, piperic acid, and piperonal were � 7.28, � 10.32, � 10.72, and � 9.57 kcal/mol, respectively. The results of ITC and molecular modeling experiments suggested that piperonylic acid and piperonal exhibit stronger binding at the active site than piperine does. From the presented studies, it could be concluded that derivatives of piperine may be of higher significance than piperine for certain medicinal applications, implicating (Ayurvedic) fermented herbal drugs with piperine in them. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text