The genome size of clusterbean (Cyamopsis tetragonoloba) is significantly smaller compared to its wild relatives as estimated by flow cytometry
| Autor: | Kishor Gaikwad, Tilak Raj Sharma, Priya Sharma, S. V. Amitha Mithra, Sandhya, Nagendra K. Singh, Swati Saxena, Ramavtar Sharma, Amolkumar U. Solanke, Anshika Tyagi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cyamopsis Guar Genomics Biology Genome 03 medical and health sciences 0302 clinical medicine Sativum Genome Size Botany Genetics Genome size Genetic diversity Oryza sativa food and beverages General Medicine Flow Cytometry biology.organism_classification Hypocotyl Plant Leaves 030104 developmental biology Organ Specificity 030220 oncology & carcinogenesis Genome Plant |
| Zdroj: | Gene. 707:205-211 |
| ISSN: | 0378-1119 |
| Popis: | Clusterbean (C. tetragonoloba) is an important, leguminous vegetable and industrial crop with vast genetic diversity but meager genetic, cytological and genomic information. In the present study, an optimized procedure of flow cytometry was used to estimate the genome size of three clusterbean species, represented by C. tetragonoloba (cv. RGC-936) and two wild relatives (C. serreta and C. senegalensis). For accurate estimation of genomic content, singlet G0/G1 populations of multiple tissues such as leaves, hypocotyl, and matured seeds were determined and used along with three different plant species viz. Pisum sativum (as primary), Oryza sativa, and Glycine max (secondary), as external and internal reference standards. Seed tissue of the test sample and G. max provided the best estimate of nuclear DNA content in comparison to other sample tissues and reference standards. The genome size of C. tetragonoloba was detemined at 580.9 ± 0.02 Mbp (1C), while that of C. serreta and C. senegalensis was estimated at 979.6 ± 0.02 Mbp (1C) and 943.4 ± 0.03 Mbp (1C), respectively. Thus, the wild relatives harbor, nearly double the genome content of the cultivated cluster bean. Findings of this study will enrich genomic database of the legume family and can serve as the starting point for clusterbean evolutionary and genomics studies. |
| Databáze: | OpenAIRE |
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