Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface
| Autor: | Neil Dani, Vanessa I. Flores, Tomas Kirchhausen, Claire Wyart, Christopher A. Deister, Ryann M. Fame, Eric M. Klein, Frederick B. Shipley, Jin Cui, Yong Zhang, Morgan L. Shannon, Kangmin He, Cameron Sadegh, Emily Jang, Michael J. Holtzman, Mark L. Andermann, Christopher I. Moore, Glenn J. Goldey, Huixin Xu, Thomas Kishkovich, Joshua P. Head, Maria K. Lehtinen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty chemistry.chemical_element Motility Calcium Epithelium Article Mice 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Immune system medicine Animals Secretion Cerebrospinal Fluid business.industry General Neuroscience Optical Imaging Serotonin Receptor Agonists 030104 developmental biology medicine.anatomical_structure chemistry Choroid Plexus Choroid plexus Serotonin business 030217 neurology & neurosurgery |
| Zdroj: | Neuron |
| ISSN: | 0896-6273 |
| Popis: | The choroid plexus (ChP) epithelium is a source of secreted signaling factors in cerebrospinal fluid (CSF) and a key barrier between blood and brain. Here, we develop imaging tools to interrogate these functions in adult lateral ventricle ChP in whole-mount explants and in awake mice. By imaging epithelial cells in intact ChP explants, we observed calcium activity and secretory events that increased in frequency following delivery of serotonergic agonists. Using chronic two-photon imaging in awake mice, we observed spontaneous subcellular calcium events as well as strong agonist-evoked calcium activation and cytoplasmic secretion into CSF. Three-dimensional imaging of motility and mobility of multiple types of ChP immune cells at baseline and following immune challenge or focal injury revealed a range of surveillance and defensive behaviors. Together, these tools should help illuminate the diverse functions of this understudied body-brain interface. |
| Databáze: | OpenAIRE |
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