Structural investigations and biological activity of inositol sphingophospholipids from Phytophthora capsici
| Autor: | P. Mas, Catherine E. Costello, Olivier Lhomme, Maud Bruneteau, Paul-Michel Molot, Georges Michel, Anne Dell, Philip R. Tiller |
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| Přispěvatelé: | Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Massachussetts Institute of Technology, Partenaires INRAE, Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Imperial College London |
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Phytophthora
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences Ceramide Chromatography Gas Ceramides 010402 general chemistry Tandem mass spectrometry Mass spectrometry 01 natural sciences Biochemistry Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Inositol Mycelium 030304 developmental biology Sphingolipids 0303 health sciences Chromatography biology food and beverages biology.organism_classification 0104 chemical sciences [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Chytridiomycota Phytophthora capsici chemistry Phosphodiester bond lipids (amino acids peptides and proteins) Chromatography Thin Layer Docosenoic Acid |
| Zdroj: | European Journal of Biochemistry European Journal of Biochemistry, Wiley, 1990, 191, pp.203-209. ⟨10.1111/j.1432-1033.1990.tb19111.x⟩ |
| ISSN: | 0014-2956 1432-1327 |
| DOI: | 10.1111/j.1432-1033.1990.tb19111.x⟩ |
| Popis: | International audience; Inositol sphingophospholipids that protect pepper (Capsicum annuum c.v. Yolo Wonder) against pathogen have been isolated by chromatographic methods from the mycelium of Phytophthora capsici. The structure of the major compound was determined by chemical methods and mass spectrometry. Phosphodiester bond cleavage of the phospholipid by mild alkaline hydrolysis liberated a ceramide which contained a C16-sphingosine. This longchain base was identified by gas chromatography and mass spectrometry of its trimethylsilyl derivative. One of the amide-linked fatty acids was found to be 4-hydroxy-2 docosenoic acid. Fast-atom-bombardment mass spectrometry and fast-atom-bombardment collison-induced tandem mass spectrometry were used to characterize the ceramide as N(4-hydroxy-2-docosenoyl)C16-sphingosine. These sphingolipids have a protective effect on cotyledons of young peppers against necrotic lesions induced by the pathogen P. capsici. |
| Databáze: | OpenAIRE |
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