Design of orthogonal regulatory systems for modulating gene expression in plants
| Autor: | Michael S. Belcher, Dominique Loqué, Patrick M. Shih, Khanh M. Vuu, Mitchell G. Thompson, Amanda Agosto Ramos, Nasim Mansoori, Henrik Vibe Scheller, Andy Zhou |
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| Rok vydání: | 2020 |
| Předmět: |
Transgene
Arabidopsis Gene Expression Nicotiana benthamiana Computational biology Saccharomyces 03 medical and health sciences Gene Expression Regulation Plant Tobacco Gene expression Arabidopsis thaliana Gene Regulatory Networks Regulatory Elements Transcriptional Promoter Regions Genetic Molecular Biology Gene 030304 developmental biology Regulation of gene expression 0303 health sciences biology 030302 biochemistry & molecular biology Rational design food and beverages Promoter Cell Biology biology.organism_classification Transcription Factors |
| Zdroj: | Nature Chemical Biology. 16:857-865 |
| ISSN: | 1552-4469 1552-4450 |
| Popis: | Agricultural biotechnology strategies often require the precise regulation of multiple genes to effectively modify complex plant traits. However, most efforts are hindered by a lack of characterized tools that allow for reliable and targeted expression of transgenes. We have successfully engineered a library of synthetic transcriptional regulators that modulate expression strength in planta. By leveraging orthogonal regulatory systems from Saccharomyces spp., we have developed a strategy for the design of synthetic activators, synthetic repressors, and synthetic promoters and have validated their use in Nicotiana benthamiana and Arabidopsis thaliana. This characterization of contributing genetic elements that dictate gene expression represents a foundation for the rational design of refined synthetic regulators. Our findings demonstrate that these tools provide variation in transcriptional output while enabling the concerted expression of multiple genes in a tissue-specific and environmentally responsive manner, providing a basis for generating complex genetic circuits that process endogenous and environmental stimuli. |
| Databáze: | OpenAIRE |
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