Oral delivery of fish oil in oil-in-water nanoemulsion : development, colloidal stability and modulatory effect on in vivo inflammatory induction in mice

Autor: João Paulo Figueiró Longo, José Athayde Vasconcelos Morais, Débora Silva Santos, Ísis A.C. Vanderlei, Osmindo Rodrigues Pires Júnior, Alexandre S. Santos, Sebastião William da Silva, Márcia Renata Mortari, Ricardo Bentes Azevedo, Luis Alexandre Muehlmann, Jaqueline Rodrigues da Silva
Rok vydání: 2020
Předmět:
0301 basic medicine
Anti-Inflammatory Agents
Administration
Oral
Mice
0302 clinical medicine
Drug Stability
Oral administration
Edema
Food science
chemistry.chemical_classification
Mice
Inbred BALB C
Chemistry
General Medicine
Fish oil
Drug Combinations
Eicosapentaenoic Acid
Neutrophil Infiltration
030220 oncology & carcinogenesis
Nanoemulsão de óleo de peixe
Emulsions
Female
Fish oil nanoemulsion
food.ingredient
Tratamento
Docosahexaenoic Acids
Drug Compounding
RM1-950
Absorption (skin)
03 medical and health sciences
Nutraceutical
food
In vivo
Animals
Oral absorption
Viability assay
Particle Size
Inflammation
Pharmacology
Macrophages
Sunflower oil
Water
Fatty acid
Disease Models
Animal
Inflamação
RAW 264.7 Cells
030104 developmental biology
Gastric Emptying
Nanoparticles
Therapeutics. Pharmacology
Inflammatory treatment
Zdroj: Repositório Institucional da UnB
Universidade de Brasília (UnB)
instacron:UNB
Biomedicine & Pharmacotherapy, Vol 133, Iss, Pp 110980-(2021)
Popis: To improve the oral absorption of fish oil and test its anti-inflammatory effect, a fish oil nanoemulsion was developed using cis-4,7,10,13,16,19-docosahexaenoic fatty acid as a biomarker for oral administration. The colloidal stability tests of the fish oil nanoemulsion showed an average size of 155.44 nm ± 6.46 (4 °C); 163.04 nm ± 9.97 (25 °C) and polydispersity index 0.22 ± 0.02 (4 °C), 0.21 ± 0.02 (25 °C), indicating systems with low polydispersity and stable droplets. The fish oil nanoemulsion did not alter the cell viability of the RAW 264.7 macrophages and, at a concentration of 0.024 mg/mL, was kinetically incorporated into the cells after 18 h of contact. The nanoemulsion was maintained in the gastrointestinal region for a significantly shorter period of time (p ≤ 0.05) compared to the intake of fish oil in free form. Inflammatory tests demonstrated that nanoemulsion and fish oil showed less (p ≤ 0.05) neutrophil infiltration after 24h of sepsis induction and there was a significant reduction (p ≤ 0.05) in the volume of paw edema in female adult Balb/c mice who received the nanoemulsion diet compared to the other experimental groups (control, formalin, fish oil and sunflower oil). These results indicate that the fish oil nanoemulsion was significantly effective in the dietary conditions tested here, presenting satisfactory responses in the modulation of inflammatory disorders, demonstrating interesting and beneficial nutraceutical effects.
Databáze: OpenAIRE
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