Coloured semi-reflective thin films for biomass-hydrolyzing enzyme detection
| Autor: | Aurélie Guyomard-Lack, Céline Moreau, Fabrice Cousin, Estelle Bonnin, Carole Cerclier, Bruno Jean, Bernard Cathala, Nadège Beury |
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| Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), INRA, Region Pays de la Loire |
| Rok vydání: | 2011 |
| Předmět: |
Materials science
POLYELECTROLYTE MULTILAYERS genetic structures XYLOGLUCAN Polymers Biomass 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Hydrolysis Cellulase [SDV.IDA]Life Sciences [q-bio]/Food engineering General Materials Science ASSAY [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Thin film Cellulose Glucans chemistry.chemical_classification NANOMOLE RANGE biology Mechanical Engineering REDUCING SUGARS 021001 nanoscience & nanotechnology Enzyme assay 0104 chemical sciences Enzymes Xyloglucan Enzyme chemistry Chemical engineering Mechanics of Materials biology.protein Nanoparticles Colorimetry Xylans Self-assembly Naked eye sense organs 0210 nano-technology |
| Zdroj: | Advanced Materials Advanced Materials, Wiley-VCH Verlag, 2011, 23 (33), pp.3791-+. ⟨10.1002/adma.201101971⟩ Advanced Materials, 2011, 23 (33), pp.3791-+. ⟨10.1002/adma.201101971⟩ |
| ISSN: | 1521-4095 0935-9648 |
| DOI: | 10.1002/adma.201101971⟩ |
| Popis: | International audience; A new enzymatic activity detection assay based on colour change of the semi-reflective films is presented. The method is based on the preparation of multilayered thin films of controlled thickness obtained by sequential deposition of cellulose nanocrystals and xyloglucan. The hydrolysis of the films leads to a decrease in layer thickness that enables to detect enzyme activity, to the naked eye, from the resulting colour changes in a span of few minutes. The method allows direct, fast, highly sensitive, and easy-to-use characterization of enzymatic activities. |
| Databáze: | OpenAIRE |
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