Comparative [18F]FDG and [18F]DPA714 PET imaging and time-dependent changes of brown adipose tissue in tumor-bearing mice
| Autor: | Haiyan Zhao, Chao Ren, Chao Fu, Lianfang Zheng, Li Huo, Jie Ding, Jiantao Ba, Na Niu, Zhixin Hao, Xuezhu Wang, Haiqun Xing |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Histology Physiology White adipose tissue Mitochondrion Diseases of the endocrine glands. Clinical endocrinology pet In vivo Internal medicine energy metabolism Brown adipose tissue medicine Translocator protein QP1-981 QH573-671 biology medicine.diagnostic_test tumour Chemistry brown adipose tissue Transporter [18f]dpa714 Cell Biology RC648-665 Endocrinology medicine.anatomical_structure Positron emission tomography Tumor progression biology.protein Cytology [18f]fdg |
| Zdroj: | Adipocyte, Vol 9, Iss 1, Pp 542-549 (2020) |
| ISSN: | 2162-397X 2162-3945 |
| DOI: | 10.1080/21623945.2020.1814546 |
| Popis: | Brown adipose tissue (BAT) is important in monitoring energy homeostasis and cancer cachexia. Different from white adipose tissue, BAT is characterized by the presence of a large number of mitochondria in adipocytes. Translocator protein 18 kDa (TSPO), a critical transporter, is expressed in the outer membrane of mitochondria. We speculated that [18F]DPA714, a specific TSPO tracer, may monitor BAT activity in tumor-bearing mice in vivo. We first analyzed the radioactive uptake of positron emission tomography (PET) tracers in BAT of CT26 xenograft mice with 18F-fluorodeoxyglucose ([18F]FDG) and [18F]DPA714. We also studied the BAT uptake of [18F]DPA714 in CT26, A549 and LLC tumor models. The dynamic distribution of [18F]FDG is quite variable among animals, even in mice of the same tumor model (%ID/g-mean: mean ± SDM, 8.61 ± 8.90, n = 16). Contrarily, [18F]DPA714 produced high-quality and stable BAT imaging in different tumor models and different animals of the same model. Interestingly, %ID/g-mean of [18F]DPA714 in BAT was significantly higher on day 26 than that on day 7 in CT26 xenograft model. Taken together, these results strongly indicate the potential feasibility of [18F]DPA714 PET imaging in investigating BAT and energy metabolism during tumor progression in preclinical and clinical study. |
| Databáze: | OpenAIRE |
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