Regulation of intracellular calcium in dispersed fat body trophocytes of the cockroach, Periplaneta americana, by hypertrehalosemic hormone
| Autor: | J.E. Steele, D. Sun |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Calmodulin biology Physiology chemistry.chemical_element Calcium biology.organism_classification Calcium in biology chemistry.chemical_compound Cytosol Endocrinology chemistry Insect Science Internal medicine biology.protein medicine Phorbol Extracellular Protein kinase C Periplaneta |
| Zdroj: | Journal of insect physiology. 47(12) |
| ISSN: | 1879-1611 |
| Popis: | Incubation of trophocytes from dissaggregated fat body of Periplaneta americana with either of the hypertrehalosemic hormones, HTH-I or HTH-II, leads to an increase in the cytosolic concentration of Ca(2+) from approximately 80 to approximately 310nM with a rise time of approximately 110s. The Ca(2+) concentration then declines to the resting level during the ensuing 5min. In the absence of extracellular Ca(2+) the increase in [Ca(2+)](i) due to HTH is limited to approximately 100nM. The calmodulin inhibitors calmidazolium and W-7 also limit to a similar degree the ability of HTH to increase [Ca(2+)](i). Phorbol 12-myristate 13-acetate, an activator of protein kinase C, was shown to block Ca(2+) entry through the plasma membrane. Additional evidence to support the view that HTH enhances Ca(2+) influx has been obtained by measuring the quenching of fura-2 fluorescence when Ca(2+) is replaced with Mn(2+). |
| Databáze: | OpenAIRE |
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