Proteasome activator REGγ promotes inflammation in Leydig cells via IkBε signaling
| Autor: | Tingmei Huang, Shihui Shen, Yunfei Xu, Guanghui Hu, Hui Chen, Lei Li, Bisheng Huang, Tiancheng Xie |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Proteasome Endopeptidase Complex endocrine system Cell Apoptosis Inflammation Autoantigens Cell Line 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Testis Genetics medicine Animals nuclear factor light-chain-enhancer of activated B cells Chemistry NF-kappa B Leydig Cells Articles General Medicine Cell cycle I-kappa B Kinase Cell biology Mice Inbred C57BL Disease Models Animal Proteasome Activator Complex Subunit 3 030104 developmental biology medicine.anatomical_structure Proteasome Cell culture 030220 oncology & carcinogenesis Proteolysis medicine.symptom Signal transduction nuclear factor light-chain-enhancer of activated B cells inhibitor ε proteasome activator complex subunit 3 Signal Transduction |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2019.4115 |
| Popis: | The development of testicular inflammation affects the normal male reproductive function. The proteasome activator complex subunit 3 (REGγ) has been suggested to regulate experimental colitis. However, to the best of our knowledge, a potential association between REGγ and testicular inflammation has not been demonstrated. The present study successfully established inflammatory models in C57 mice, primary Leydig cells and the TM3 cell line. It was observed that the absence of REGγ conveyed a significantly protective effect toward testosterone secretion in Leydig cells. REGγ deficiency significantly decreased the expression levels of phosphorylated transcription factor p65 and inflammatory factors in testis tissues, primary Leydig cells and the TM3 cell line. Inflammation also upregulated the expression levels of REGγ. Furthermore, the degradation of the nuclear factor light‑chain‑enhancer of activated B cells (NF‑κB) inhibitor ε (IkBε) signaling pathway regulated REGγ and NF‑κB expression. Double knockdown of REGγ and IkBε restored the response in wild‑type cells to LPS‑induced inflammation. In summary, these results demonstrated that REGγ regulates NF‑κB activity by specifically degrading IkBε to regulate inflammation in testicular Leydig cells. |
| Databáze: | OpenAIRE |
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