Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA
| Autor: | Pierre V. Vignais, Marianne Tardif, François Boulay, Laurence Brouchon |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
G protein
Molecular Sequence Data Biophysics Peptide Biology Transfection Biochemistry Cell Line Complementary DNA Animals Humans Amino Acid Sequence Cloning Molecular Receptors Immunologic Receptor Molecular Biology Gene Library chemistry.chemical_classification Expression vector COS cells Formyl peptide receptor Base Sequence Superoxide Dismutase cDNA library DNA Cell Biology Receptors Formyl Peptide Molecular biology N-Formylmethionine Leucyl-Phenylalanine chemistry |
| Zdroj: | Biochemical and Biophysical Research Communications. 168:1103-1109 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(90)91143-g |
| Popis: | N-formyl-methionyl peptides are powerful chemoattractants which bind to specific receptors on the neutrophil plasma membrane. A cDNA library from HL-60 cells, differentiated into granulocytes highly responsive to N-formyl-methionyl peptides, was constructed in the COS cell expression vector CDM8. A cDNA clone was isolated that conferred to COS cells the ability to bind a new and highly efficient hydrophilic derivative of N-formyl-Met-Leu-Phe-Lys. The transfected COS cells displayed two classes of binding sites with Kd values of 1–5nM and 5–10 nM, respectively. The cDNA was 1.9 kb long with a 1050 bp open reading frame encoding a 350 residue protein. The hydropathy plot analysis revealed seven hydrophobic segments, a pattern quite similar to that of G protein-coupled receptors. |
| Databáze: | OpenAIRE |
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