Transcriptome Profiling Reveals the EanI/R Quorum Sensing Regulon in Pantoea Ananatis LMG 2665
| Autor: | Siphathele Sibanda, Teresa A. Coutinho, Collins Kipngetich Tanui, Divine Yufetar Shyntum, Lucy N. Moleleki, Stanford Kwenda |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
LMG 2665T lcsh:QH426-470 030106 microbiology Mutant acyl homoserine lactones Article Transcriptome Pantoea ananatis quorum sensing regulon RNA-seq 03 medical and health sciences Gene expression Genetics Gene Genetics (clinical) Chemistry Biofilm food and beverages lcsh:Genetics Quorum sensing Regulon Biochemistry Membrane protein |
| Zdroj: | Genes Genes; Volume 9; Issue 3; Pages: 148 Genes, Vol 9, Iss 3, p 148 (2018) |
| ISSN: | 2073-4425 |
| Popis: | Pantoea ananatis LMG 2665T synthesizes and utilizes acyl homoserine lactones (AHLs) for signalling. The complete set of genes regulated by the EanI/R quorum sensing (QS) system in this strain is still not fully known. In this study, RNA-sequencing (RNA-seq) was used to identify the EanI/R regulon in LMG 2665T. Pairwise comparisons of LMG 2665T in the absence of AHLs (Optical density (OD)600 = 0.2) and in the presence of AHLs (OD600 = 0.5) were performed. Additionally, pairwise comparisons of LMG 2665T and its QS mutant at OD600 = 0.5 were undertaken. In total, 608 genes were differentially expressed between LMG 2665T at OD600 = 0.5 versus the same strain at OD600 = 0.2 and 701 genes were differentially expressed between LMG 2665T versus its QS mutant at OD600 = 0.5. A total of 196 genes were commonly differentially expressed between the two approaches. These constituted approximately 4.5% of the whole transcriptome under the experimental conditions used in this study. The RNA-seq data was validated by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Genes found to be regulated by EanI/R QS were those coding for redox sensing, metabolism, flagella formation, flagella dependent motility, cell adhesion, biofilm formation, regulators, transport, chemotaxis, methyl accepting proteins, membrane proteins, cell wall synthesis, stress response and a large number of hypothetical proteins. The results of this study give insight into the genes that are regulated by the EanI/R system in LMG 2665T. Functional characterization of the QS regulated genes in LMG 2665T could assist in the formulation of control strategies for this plant pathogen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|