Isolation and characterization of exosomes from adipose tissue‐derived mesenchymal stem cells
| Autor: | Eliezer Navarro-Ramírez, Laura Gutiérrez-Velasco, María Luisa González-Fernández, Vega Villar-Suárez, Elsa González-Cubero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Histology Adipose tissue Biology Exosomes Exosome Extracellular vesicles 03 medical and health sciences 0302 clinical medicine microRNA Humans Molecular Biology Ecology Evolution Behavior and Systematics Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Isolation (microbiology) Original Papers Microvesicles Cell biology 030104 developmental biology Adipose Tissue Cell culture Anatomy 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | J Anat |
| Popis: | Mesenchymal stem cells (MSCs) are the subject of intense research as they are a potential therapeutic tool for several clinical applications. The new MSCs action models are focused on the use of MSC-derived secretome which contains several growth factors, cytokines, microRNAs, and extracellular vesicles such as exosomes. Exosomes have recently emerged as a component with great potential involved as mediators in cellular communication. The isolation and identification of exosomes has made it possible for them to be used in cell-free therapies. The purposes of this study are: (i) to detect exosomes released into adipose-derived MSC conditioned cell culture medium, (ii) to identify exosome morphology, and (iii) to carry out a complete characterization of said exosomes. Moreover, it is aimed at determining which method for exosome isolation would be best to use. Precipitation has been identified as a highly useful method of exosome isolation since it provides higher efficiency and purity values than other methods. A broad characterization of the exosomes present in the MSC-conditioned medium was also carried out. This work fills a gap in the existing literature on bioactive molecules which have attracted a great deal of interest due to their potential use in cellular therapies. |
| Databáze: | OpenAIRE |
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