Rapid and sensitive assay for the phytotoxin rhizobitoxine
| Autor: | N K Peters, X Ruan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Salmonella typhimurium
Rhizobiaceae Ecology Pseudomonas fungi food and beverages Lyases Phytotoxin Biology biochemical phenomena metabolism and nutrition biology.organism_classification Applied Microbiology and Biotechnology Bradyrhizobium Microbiology Propanolamines Pseudomonadales bacteria Bacteria Food Science Biotechnology Pseudomonadaceae Bradyrhizobium japonicum Research Article |
| Popis: | Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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