Angiotensin II receptor expression following intestinal transplantation in mice
| Autor: | Emma, Lars Fändriks, Anders Aneman, G. Dindelegan, Michael Olausson, Sara Ewert, Michael Vieth |
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| Rok vydání: | 2005 |
| Předmět: |
Graft Rejection
Male medicine.medical_specialty Angiotensin receptor Ratón Receptor expression Blotting Western Gene Expression Biology Receptor Angiotensin Type 2 Receptor Angiotensin Type 1 Renin-Angiotensin System Mice Western blot Internal medicine Renin–angiotensin system medicine Animals Receptor Mice Inbred BALB C medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Graft Survival Angiotensin II Up-Regulation Transplantation Mice Inbred C57BL surgical procedures operative Endocrinology Jejunum Surgery |
| Zdroj: | The Journal of surgical research. 135(1) |
| ISSN: | 0022-4804 |
| Popis: | Background To further improve the success rate of intestinal transplantation there is a need to find early appearing indicators of rejection. The specific aim of this study was to compare Angiotensin (Ang) II type 1 receptor and Ang II type 2 receptor expression in relation to histological signs of rejection. Methods Mice of the C57BL6 strain with syngeneic intestinal grafts were compared to mice subjected to allogeneic intestinal transplantation with BalbC strain as donors. Local expression of Ang II type 1 and 2 receptor was evaluated using rt-PCR and Western blot and compared to histological picture in grafts and native intestine. Results The Ang II type 2 receptor protein expression was markedly up-regulated in the allogeneically transplanted graft from day 1 postoperatively. Histological signs of rejection were not seen until day 6. Conclusion Intestinal allograft transplantation in mice is associated with a marked up-regulation of the Ang II type 2 receptor. However, the detailed role of the renin-angiotensin system in the immune rejection following intestinal transplantation remains to be clarified. |
| Databáze: | OpenAIRE |
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