Transforming growth factor β1 induces IL-1 receptor antagonist production and gene expression in rat vascular smooth muscle cells
Autor: | Ettore Porreca, Franco Cuccurullo, Giovanna Baccante, Antonio Angelini, Marcella Reale, Maria Luisa Castellani, Concetta Di Febbo |
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Rok vydání: | 1998 |
Předmět: |
medicine.medical_specialty
Vascular smooth muscle Arteriosclerosis medicine.drug_class Gene Expression Receptors Interleukin-1 Biology Receptor antagonist Muscle Smooth Vascular Rats Proinflammatory cytokine Endocrinology Transforming Growth Factor beta Cell culture Internal medicine Gene expression medicine Animals RNA Messenger Cardiology and Cardiovascular Medicine Receptor Autocrine signalling Cells Cultured Transforming growth factor |
Zdroj: | Atherosclerosis. 136:377-382 |
ISSN: | 0021-9150 |
DOI: | 10.1016/s0021-9150(97)00240-2 |
Popis: | Atherosclerosis is an inflammatory-fibroproliferative process that may represent a possible milieu in which transforming growth factor-beta (TGF-beta) can be involved. Vascular smooth muscle cells (VSMC) may represent a source or a target of a large number of growth factors and proinflammatory cytokines, including interleukin-1 and its receptor antagonist (IL-1Ra). We tested the effect of TGF-beta1, on IL-1Ra production and gene expression in rat VSMC cultures. We found a significant dose (3-30 ng/ml) and time-dependent (0-48 h) increase in IL-1Ra immunoactivity in the supernatant of conditioned medium and cell lysates. The maximal effect was observed with TGF-beta at 30 ng/ml and after 24 h incubation time, respect to untreated cells (320 +/- 26 vs. 211 +/- 20 pg/ml; P < 0.01). Furthermore, TGF-beta1 induced an increased mRNA expression which began at 2 h and peaked at 18 h incubation time (about a 6-fold increase with respect to unstimulated cells). The effect of TGF-beta1 on IL-1Ra production was completely inhibited by an anti-IL-1beta antibody (10 microg/ml) (from 320 +/- 81 to 181 +/- 46 pg/ml). These experiments suggest that TGF-beta1, potentially produced in the vascular wall during atherogenesis, may play a pathophysiological role in the autocrine control of IL-1 actions, via VSMC IL-1Ra production. |
Databáze: | OpenAIRE |
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