TSLP Expression: Analysis with a ZsGreen TSLP Reporter Mouse
Autor: | Joy A. Williams, Mark C. Udey, Stephen F. Porcella, Xi Chen, William E. Paul, Ilkka S. Junttila, Cedric Dewas, Jay T. Linton, Daniel E. Sturdevant, Tetsuya Honda, Lionel Feigenbaum, Lily Koo |
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Rok vydání: | 2015 |
Předmět: |
Keratinocytes
Thymic stromal lymphopoietin Recombinant Fusion Proteins medicine.medical_treatment Transgene Genetic Vectors Immunology Gene Expression Mice Transgenic Biology Lymphocyte Activation Article Mice Th2 Cells Thymic Stromal Lymphopoietin Genes Reporter Gene Order medicine Animals Immunology and Allergy Mast Cells Interleukin 4 Cholecalciferol Regulation of gene expression Interleukin-13 Tumor Necrosis Factor-alpha Epithelial Cells Dendritic Cells Th1 Cells Basophils Cell biology Cytokine medicine.anatomical_structure Gene Expression Regulation Interleukin 13 Cytokines Tumor necrosis factor alpha Interleukin-4 Keratinocyte |
Zdroj: | The Journal of Immunology. 194:1372-1380 |
ISSN: | 1550-6606 0022-1767 |
DOI: | 10.4049/jimmunol.1400519 |
Popis: | Thymic stromal lymphopoietin (TSLP) is a type I cytokine that plays a central role in induction of allergic inflammatory responses. Its principal targets have been reported to be dendritic cells and/or CD4 T cells; epithelial cells are a principal source. We report in this study the development of a reporter mouse (TSLP-ZsG) in which a ZsGreen (ZsG)-encoding construct has been inserted by recombineering into a bacterial artificial chromosome immediately at the translation initiating ATG of TSLP. The expression of ZsG by mice transgenic for the recombinant BAC appears to be a faithful surrogate for TSLP expression, particularly in keratinocytes and medullary thymic epithelial cells. Limited ZsG and TSLP mRNA was observed in bone marrow–derived mast cells, basophils, and dendritic cells. Using the TSLP-ZsG reporter mouse, we show that TNF-α and IL-4/IL-13 are potent inducers of TSLP expression by keratinocytes and that local activation of Th2 and Th1 cells induces keratinocyte TSLP expression. We suggest that the capacity of TSLP to both induce Th2 differentiation and to be induced by activated Th2 cells raises the possibility that TSLP may be involved in a positive feedback loop to enhance allergic inflammatory conditions. |
Databáze: | OpenAIRE |
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