Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging

Autor: François Guillemot, Vladislav I. Korobeynyk, Fritjof Helmchen, Merit Kruse, Lachlan Harris, Baptiste N. Jaeger, Sara Bottes, Sebastian Jessberger, Gregor-Alexander Pilz, Izaskun Mallona, David J. Jörg, John Darby Cole, Benjamin D. Simons
Přispěvatelé: Jaeger, Baptiste N [0000-0001-8728-797X], Pilz, Gregor-Alexander [0000-0002-6161-4976], Jörg, David J [0000-0001-5960-0260], Cole, John Darby [0000-0002-3615-8702], Mallona, Izaskun [0000-0002-2853-7526], Helmchen, Fritjof [0000-0002-8867-9569], Simons, Benjamin D [0000-0002-3875-7071], Jessberger, Sebastian [0000-0002-0056-8275], Apollo - University of Cambridge Repository, University of Zurich, Jessberger, Sebastian
Jazyk: angličtina
Předmět:
0301 basic medicine
Male
Intravital Microscopy
Population
Hippocampus
610 Medicine & health
Mice
Transgenic

Nerve Tissue Proteins
Biology
Zinc Finger Protein GLI1
Article
03 medical and health sciences
Mice
0302 clinical medicine
Single-cell analysis
Neural Stem Cells
Basic Helix-Loop-Helix Transcription Factors
Animals
Cell Lineage
10064 Neuroscience Center Zurich
education
reproductive and urinary physiology
Homeodomain Proteins
education.field_of_study
10242 Brain Research Institute
General Neuroscience
Gene Expression Profiling
Neurogenesis
2800 General Neuroscience
Neural stem cell
10124 Institute of Molecular Life Sciences
Metallothionein 3
nervous system diseases
Nerve Regeneration
Mice
Inbred C57BL

ASCL1
030104 developmental biology
Microscopy
Fluorescence
Multiphoton

nervous system
570 Life sciences
biology
Female
Stem cell
biological phenomena
cell phenomena
and immunity

Single-Cell Analysis
Neuroscience
030217 neurology & neurosurgery
Intravital microscopy
Zdroj: Nature neuroscience
Nature Neuroscience
ISSN: 1546-1726
1097-6256
DOI: 10.1038/s41593-020-00759-4
Popis: Neural stem cells (NSCs) generate neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of individual NSCs within the adult brain remains unclear. We used two-photon microscopy and followed NSCs that were genetically labeled through conditional recombination driven by the regulatory elements of the stem cell-expressed genes GLI family zinc finger 1 (Gli1) or achaete-scute homolog 1 (Ascl1). Through intravital imaging of NSCs and their progeny, we identify a population of Gli1-targeted NSCs showing long-term self-renewal in the adult hippocampus. In contrast, once activated, Ascl1-targeted NSCs undergo limited proliferative activity before they become exhausted. Using single-cell RNA sequencing, we show that Gli1- and Ascl1-targeted cells have highly similar yet distinct transcriptional profiles, supporting the existence of heterogeneous NSC populations with diverse behavioral properties. Thus, we here identify long-term self-renewing NSCs that contribute to the generation of new neurons in the adult hippocampus.
Databáze: OpenAIRE
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