Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging
Autor: | François Guillemot, Vladislav I. Korobeynyk, Fritjof Helmchen, Merit Kruse, Lachlan Harris, Baptiste N. Jaeger, Sara Bottes, Sebastian Jessberger, Gregor-Alexander Pilz, Izaskun Mallona, David J. Jörg, John Darby Cole, Benjamin D. Simons |
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Přispěvatelé: | Jaeger, Baptiste N [0000-0001-8728-797X], Pilz, Gregor-Alexander [0000-0002-6161-4976], Jörg, David J [0000-0001-5960-0260], Cole, John Darby [0000-0002-3615-8702], Mallona, Izaskun [0000-0002-2853-7526], Helmchen, Fritjof [0000-0002-8867-9569], Simons, Benjamin D [0000-0002-3875-7071], Jessberger, Sebastian [0000-0002-0056-8275], Apollo - University of Cambridge Repository, University of Zurich, Jessberger, Sebastian |
Jazyk: | angličtina |
Předmět: |
0301 basic medicine
Male Intravital Microscopy Population Hippocampus 610 Medicine & health Mice Transgenic Nerve Tissue Proteins Biology Zinc Finger Protein GLI1 Article 03 medical and health sciences Mice 0302 clinical medicine Single-cell analysis Neural Stem Cells Basic Helix-Loop-Helix Transcription Factors Animals Cell Lineage 10064 Neuroscience Center Zurich education reproductive and urinary physiology Homeodomain Proteins education.field_of_study 10242 Brain Research Institute General Neuroscience Gene Expression Profiling Neurogenesis 2800 General Neuroscience Neural stem cell 10124 Institute of Molecular Life Sciences Metallothionein 3 nervous system diseases Nerve Regeneration Mice Inbred C57BL ASCL1 030104 developmental biology Microscopy Fluorescence Multiphoton nervous system 570 Life sciences biology Female Stem cell biological phenomena cell phenomena and immunity Single-Cell Analysis Neuroscience 030217 neurology & neurosurgery Intravital microscopy |
Zdroj: | Nature neuroscience Nature Neuroscience |
ISSN: | 1546-1726 1097-6256 |
DOI: | 10.1038/s41593-020-00759-4 |
Popis: | Neural stem cells (NSCs) generate neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of individual NSCs within the adult brain remains unclear. We used two-photon microscopy and followed NSCs that were genetically labeled through conditional recombination driven by the regulatory elements of the stem cell-expressed genes GLI family zinc finger 1 (Gli1) or achaete-scute homolog 1 (Ascl1). Through intravital imaging of NSCs and their progeny, we identify a population of Gli1-targeted NSCs showing long-term self-renewal in the adult hippocampus. In contrast, once activated, Ascl1-targeted NSCs undergo limited proliferative activity before they become exhausted. Using single-cell RNA sequencing, we show that Gli1- and Ascl1-targeted cells have highly similar yet distinct transcriptional profiles, supporting the existence of heterogeneous NSC populations with diverse behavioral properties. Thus, we here identify long-term self-renewing NSCs that contribute to the generation of new neurons in the adult hippocampus. |
Databáze: | OpenAIRE |
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