RT-qPCR for the diagnosis of the vesiculovirus Cocal virus
| Autor: | Mariana Lázaro Sales, Jenner Karlisson Pimenta dos Reis, Mariana Dall’Agnol, Marcelo Fernandes Camargos, Antônio Augusto Fonseca, Anapolino Macedo de Oliveira |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
viruses Biology Real-Time Polymerase Chain Reaction Virus 03 medical and health sciences Vesicular Stomatitis Medical microbiology Virology medicine Animals 030304 developmental biology 0303 health sciences 030306 microbiology DNA Viruses Outbreak General Medicine Vesiculovirus Cocal virus Real-time polymerase chain reaction Vesicular stomatitis Alagoas virus Foot-and-Mouth Disease Virus Foot-and-Mouth Disease RNA Viral Brazil |
| Zdroj: | Archives of virology. 165(8) |
| ISSN: | 1432-8798 |
| Popis: | Cocal virus (COCV) is one of the causative agents of vesicular stomatitis, presenting clinical signs indistinguishable from those caused by foot-and-mouth disease virus (FMDV). Therefore, the differentiation of these two viruses via laboratory diagnosis is essential. The objective of this study was to develop and validate a real-time quantitative PCR (RT-qPCR) protocol for the diagnosis of COCV directly from epithelial samples. The method developed had 97% accuracy at 3950 pfu and a repeatability error of 1.29%. RT-qPCR was able to distinguish COCV from other viruses that cause vesicular diseases, an important factor because seroneutralization may produce cross-reactivity between COCV and vesicular stomatitis Alagoas virus (VSAV). No epithelial sample originating from vesicular disease outbreaks between 2014 and 2018 in Brazil was positive for COCV. |
| Databáze: | OpenAIRE |
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