Comparative assessment of Prunus spinosa L. flower extract in non-neoplastic hepatocytes and hepatoblastoma cells

Autor: Verica Dragović-Uzelac, A. Štefanko, Marina Miletić, Ivana Kmetič, I. Elez Garofulić, I. Landeka Jurčević, Teuta Murati
Rok vydání: 2019
Předmět:
Zdroj: South African Journal of Botany. 123:36-42
ISSN: 0254-6299
DOI: 10.1016/j.sajb.2019.02.006
Popis: Prunus spinosa L. (blackthorn) flower was most recently pointed out as a valuable source of bioactive compounds that have potent anticancer properties. To test for antineoplastic activity, hepatocarcinoma cells (Hepa 1–6) and corresponding non-neoplastic hepatocytes (AML 12) were subjected to Prunus spinosa L. blossom ethanolic extract (5–200 μg mL−1). Antiproliferative activity was determined by Trypan Blue (TB) and Kenacid Blue (KB) methods after 24 and 72 h. Inhibitory effects on Hepa 1–6 proliferation were observed at all concentrations with significant inhibition at ≥ 100 μg mL−1 (p < .025–p < .001) determined with both methods. Cytotoxicity was also detected in non-neoplastic hepatocytes with significant doses above 10 μg mL−1 (p < .05–p < .001) after 72 h. Cellular viability loss after 24 h was more pronounced in non-neoplastic vs. hepatocarcinoma cells as confirmed with IC50TB values (half maximal inhibitory concentration): 59.05 μg mL−1 in AML 12 vs. 166.87 μg mL−1 in Hepa 1–6 cells and IC50KB: 72.69 vs. 172.93 μg mL−1, respectively. Prolonged incubation (72 h) resulted in almost equal toxicity in both cell lines. Prooxidative activity of blackthorn flower extract was observed in both cell lines with higher ROS formation in neoplastic cells. Cell death was primarily through necrosis. The proportion of apoptotic cells in both cultures was low: fraction of early apoptosis – 0.04–1.65% of the total cell population and fraction of late apoptosis: only 0.05–3.25%.
Databáze: OpenAIRE
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