Cerium and Yttrium Oxide Nanoparticles and Nano-selenium Produce Protective Effects Against H2O2-induced Oxidative Stress in Pancreatic Beta Cells by Modulating Mitochondrial Dysfunction
| Autor: | Asieh Hosseini, Seyed Vahid Shetab-Boushehri, Alireza Ebadollahi-Natanzi, Mona Navaei-Nigjeh, Nazanin Namazi Sarvestani, Amir Hossein Baghsheikhi, Shima Tavoosi, Mohammad Yahya Karimi, Akram Ranjbar |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Biomedical Engineering Pharmaceutical Science Apoptosis 030209 endocrinology & metabolism Pharmacology medicine.disease_cause Antioxidants Cell Line Selenium 03 medical and health sciences 0302 clinical medicine Insulin-Secreting Cells medicine Animals Insulin Uncoupling Protein 2 Yttrium Beta (finance) chemistry.chemical_classification Reactive oxygen species geography geography.geographical_feature_category Glucagon secretion Cerium Hydrogen Peroxide Glucagon Islet Mitochondria Rats Transplantation Oxidative Stress 030104 developmental biology medicine.anatomical_structure chemistry Nanoparticles Pancreas Oxidative stress |
| Zdroj: | Pharmaceutical Nanotechnology. 8:63-75 |
| ISSN: | 2211-7385 |
| DOI: | 10.2174/2211738507666191002154659 |
| Popis: | Background: Type 1 diabetes mellitus is characterized by the destruction of insulin- producing Beta cells in the pancreas. Researchers hope that islet transplantation will help to patients with insulin-dependent diabetes mellitus (IDDM). Oxidative stress is the most important challenge that beta cells face to it after isolation, and mitochondrial dysfunction is a crucial mediator in beta cells death. Hence, therapeutic approaches can shift to antioxidants through the application of nanoparticles such as cerium and yttrium oxide nanoparticles (Cer and Ytt Ox NPs) and nano-selenium (Nan Se). Objectives: This study evaluates the effects of Cer and Ytt Ox NPs and Nan Se on H2O2- induced oxidative stress in pancreatic beta cells with focus on mitochondrial dysfunction pathway. Methods: CRI-D2 beta-cell line were pretreated with Cer Ox NPs (200 µM) + Ytt Ox NPs (0.5 µg/mL) for 3 days and/or Nan Se (0.01 µM) for 1 day. Then markers of oxidative stress, mitochondrial dysfunction, insulin and glucagon secretion were measured. Results: We reported a decrease in H2O2-induced reactive oxygen species (ROS) level and glucagon secretion, and an increase in H2O2-reduced ATP/ADP ratio, MMP, as well as UCP2 protein expression, and insulin secretion by pretreatment of CRI-D2 cells with Cer and Ytt Ox NPs and/or Nan Se. Conclusion: We found maximum protective effect with Cer and Ytt Ox NPs on CRI-D2 beta-cell line exposed by H2O2 for keeping beta cells alive until transplant whereas combination of Cer and Ytt Ox NPs and Nan Se had very little protective effect in this condition. |
| Databáze: | OpenAIRE |
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