DNA-Small Molecule Chimera with Responsive Protein-Binding Ability
| Autor: | Janarthanan Jayawickramarajah, D. Calvin Harris, Xiaozhu Chu |
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| Rok vydání: | 2008 |
| Předmět: |
Models
Molecular Protein Conformation Stereochemistry Recombinant Fusion Proteins Oligonucleotides Fluorescence Polarization Plasma protein binding Biochemistry Catalysis chemistry.chemical_compound Colloid and Surface Chemistry Protein structure Trypsin Serum Albumin Carbonic Anhydrases Oligonucleotide Proteins DNA General Chemistry Phenylthiourea Small molecule Benzamidines Dissociation constant chemistry Duplex (building) Intramolecular force Nucleic Acid Conformation Fluorescein Streptavidin Thymidine |
| Zdroj: | Journal of the American Chemical Society. 130:14950-14951 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja806552c |
| Popis: | In this communication, we disclose a generalizable strategy for developing agents with regulable protein-binding ability. In particular, a responsive DNA-small molecule chimera (DC) 1 consisting of two synthetic protein-binding arms and a core oligonucleotide (ODN) domain is discussed. DC 1 can be cycled from a bidentate intramolecular quadruplex form to a monodentate duplex structure, via addition of external ODN stimuli. Importantly, these distinct secondary structures of 1 lead to significantly different protein-binding abilities, with the bidentate conformation showing a 20-fold enhancement (with a 0.8 microM dissociation constant, Kd) in trypsin-binding potency. |
| Databáze: | OpenAIRE |
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