Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress
Autor: | Masaya Satoh, Yumiko Nishimura, Yasuo Oyama, Yasuhiro Kanbara, Kazuyoshi Murakane |
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Rok vydání: | 2013 |
Předmět: |
Programmed cell death
Triclocarban Population Toxicology medicine.disease_cause chemistry.chemical_compound medicine Animals Sulfhydryl Compounds Hydrogen peroxide education Cytotoxicity Whole blood education.field_of_study Thymocytes Cell Death Hydrogen Peroxide Glutathione Molecular biology Rats Oxidative Stress chemistry Biochemistry Anti-Infective Agents Local Carbanilides Oxidative stress |
Zdroj: | The Journal of Toxicological Sciences. 38:49-55 |
ISSN: | 1880-3989 0388-1350 |
DOI: | 10.2131/jts.38.49 |
Popis: | It was recently reported that triclocarban was absorbed significantly from soap used during showering in human subjects and that its C(max) in their whole blood ranged from 23 nM to 530 nM. We revealed that a nanomolar concentration (300 nM) of triclocarban potentiated the cytotoxicity of 300 µM H(2)O(2) in rat thymocytes by using cytometric techniques with appropriate fluorescent probes. Although 300 nM triclocarban did not itself increase the population of dead cells (cell lethality), it facilitated the process of cell death induced by H(2)O(2), resulting in a further increase in the population of dead cells. Nanomolar concentrations (300 nM or higher) of triclocarban significantly decreased the cellular content of nonprotein thiol (glutathione), which has a protective role against oxidative stress. Triclocarban at 300 nM or higher increased the cell vulnerability to oxidative stress. The results may suggest that nanomolar concentration (300 nM or higher) of triclocarban affects some cellular functions although there is no evidence for adverse effects of triclocarban in humans at present. |
Databáze: | OpenAIRE |
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