Biotyping, phage typing, and O-serotyping of clinical isolates of Enterobacter cloacae
| Autor: | Mary Elizabeth Kaufmann, Merete Weischer, Hans Jørn Kolmos, Vibeke Thamdrup Rosdahl |
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| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Serotype Lipopolysaccharides Pathology and Forensic Medicine Microbiology Bacteriophage Discriminatory power Enterobacter cloacae Immunology and Allergy Humans Bacteriophages Typing Serotyping Bacteriophage Typing Phage typing Cross Infection biology Polysaccharides Bacterial Enterobacteriaceae Infections O Antigens General Medicine biology.organism_classification University hospital Enterobacteriaceae Virology |
| Zdroj: | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 101(11) |
| ISSN: | 0903-4641 |
| Popis: | The purpose of this study was to make an independent evaluation of the methods of bio-, phage-, and O-serotyping which had been used only in the laboratory of origin, and to assess the extent of possible cross-infection of Enterobacter cloacae in a Danish university hospital. The material consisted of 237 clincial isolates of E. cloacae from the clinical microbiology laboratory at Hvidovre Hospital. The typability of bio-, phage-, and serotyping was 100%, 83%, and 85%, respectively. Reproducibility of serotyping was 90% and of phage typing 96% if two major differences were allowed to differentiate between patterns. O-serotyping had the highest discriminatory power and combination of all typing methods further increased discrimination. Outbreaks of E. cloacae were not evident in clinical departments, but cross-infections from one department to another could not be completely ruled out. We concluded that the combination of bio-, phage- and O-serotyping is sufficiently discriminating and will be satisfactory in the majority of clinical situations. |
| Databáze: | OpenAIRE |
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