Two cyclin Bs are differentially modulated by glucose and sucrose during maize germination
| Autor: | Carlos E. Bravo-Alberto, Atonatiu E. Gómez-Martínez, María Fernanda Ayub-Miranda, Diana I. Romero-Sánchez, Javier Axosco-Marín, Aurora Lara-Núñez, Sonia Vázquez-Santana, Sara M. Garza-Aguilar, Jorge M. Vázquez-Ramos |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Sucrose Germination Biochemistry Zea mays 03 medical and health sciences chemistry.chemical_compound Cyclin-dependent kinase Cyclin B2 Kinase activity Cyclin B1 Sugar Cellular localization Plant Proteins 030102 biochemistry & molecular biology biology General Medicine biology.organism_classification 030104 developmental biology Glucose chemistry Seedling biology.protein Imbibition |
| Zdroj: | Biochimie. 182 |
| ISSN: | 1638-6183 |
| Popis: | Cell proliferation during seed germination is determinant for an appropriate seedling establishment. The present work aimed to evaluate the participation of two maize B-type Cyclins during germination and under the stimulus of two simple sugars: sucrose and glucose. We found out that the corresponding genes, ZmCycB1;2 and ZmCycB2;1, increased their expression at 24 h of germination, but only ZmCycB1;2 responded negatively to sugar type at the highest sugar concentration tested (120 mM). Also, CycB1;2 showed differential protein levels along germination in response to sugar, or its absence. Both CycBs interacted with CDKA;1 and CDKB1;1 by pull down assays. By an immunoprecipitation approach, it was found that each CycB associated with two CDKB isoforms (34 and 36 kDa). A higher proportion of CycB1;2-CDKB-36kDa was coincident to an increased kinase activity in the presence of sugar and particularly in glucose treatment at 36 h of imbibition. CycB1;2-CDKB activity increased in parallel to germination advance and this was dependent on sugar: glucose > sucrose > No sugar treatment. At RAM, CycB1;2 was more abundant in nuclei on Glucose at late germination; DNA-CycB1;2 colocalization was parallel to CycB1;2 inside the nucleus. Overall, results point out CycB1;2 as a player on promoting proliferation during germination by binding a specific CDKB isoform partner and changing its cellular localization to nuclei, co-localizing with DNA, being glucose a triggering signal. |
| Databáze: | OpenAIRE |
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