Biochemical and histological changes produced by sweeteners and cytarabine in the brain of young rats
| Autor: | Alberto Rojas Ochoa, Gerardo Barragán Mejía, Armando Valenzuela Peraza, Daniel Santamaría del Ángel, Hugo Juárez Olguín, Norma Osnaya Brizuela, Ernestina Hernández García, Maribel Ortiz Herrera, David Calderón Guzmán |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Antimetabolites Antineoplastic Sucrose Metabolite Dopamine Medicine (miscellaneous) Pharmacology medicine.disease_cause Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine TBARS Animals Stevia Rats Wistar Brain Chemistry Nutrition and Dietetics business.industry Cytarabine Brain Glutathione Hydroxyindoleacetic Acid Sweeteners Rats Oxidative Stress 030104 developmental biology chemistry Oxidative stress Sweetening Agents Serotonin business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Nutrición Hospitalaria v.35 n.1 2018 SciELO España. Revistas Científicas Españolas de Ciencias de la Salud instname |
| ISSN: | 1699-5198 |
| Popis: | Objective: The aim of this study was to evaluate the effect of splenda and stevia on dopamine and 5-HIAA levels, and some biomarkers of oxidative stress in the presence of cytarabine. Methods: Forty-eight young male Wistar rats each with a weight of 80 g (four weeks of age), distributed in six groups of eight animals each, were treated as follows: group 1, control (NaCl 0.9% vehicle); group 2, cytarabine (0.6 g/kg); group 3, stevia (0.6 g/kg); group 4, cytarabine + stevia; group 5, splenda; and group 6, cytarabine + splenda. Cytarabine was given intravenously (IV) while stevia and splenda were administered orally for five days, using orogastric tube. At the end of treatment, the animals were sacrificed and glucose levels in blood were measured. The brains were dissected for histological analysis and homogenated to measure levels of dopamine, lipid peroxidation (TBARS), serotonin metabolite (5-HIAA), Na+, K+ ATPase activity, and glutathione (GSH), using validated methods. Results: Sweeteners increased the glucose in animals that received cytarabine. Dopamine increased in cortex and decreased in striatum of animals that received stevia alone and combined with cytarabine. 5-HIAA decreased in striatum and cerebellum/medulla oblongata of animals that received sweeteners and cytarabine alone or combined. GSH increased in animals that received sweeteners and decreased with cytarabine. Lipoperoxidation decreased in groups that received sweeteners and cytarabine. Histopathological changes revealed marked degeneration of neuronal cells in animals treated with cytarabine. Conclusion: These results show that sweeteners as stevia or splenda may lead to the onset of unfavorable changes in dopamine and 5-HIAA. Antioxidant effects may be involved. Besides, histological changes revealed marked lesions of neuronal cells in experimental animals treated with cytarabine. |
| Databáze: | OpenAIRE |
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