Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants
| Autor: | Danuše Tarkowská, Juan Moreno, Leonardo Perez de Souza, Ralph Bock, Shreya Agrawal, Stella Kössler, Jianing Mi, Salim Al-Babili, Stephanie Ruf, Arun Sampathkumar, Anja Fröhlich, Anne Schadach, Tegan Armarego-Marriott, Veronika Turečková, Mark Aurel Schöttler |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Nicotiana tabacum β-Carotene Plant Science Photosynthetic efficiency 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant lycopene β-cyclase Tobacco Botany Nicotiana tabacum cv. Petit Havana Intramolecular Lyases Abscisic acid Carotenoid Plant Proteins chemistry.chemical_classification photosynthesis biomass transplastomic biology AcademicSubjects/SCI01210 fungi carotenoids Plant physiology food and beverages Plants Genetically Modified biology.organism_classification Research Papers phytohormones 030104 developmental biology chemistry RNAi Gibberellin Photosynthesis and Metabolism 010606 plant biology & botany Daucus carota Transplastomic plant |
| Zdroj: | Journal of Experimental Botany |
| Popis: | Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-Carotene is generated from lycopene by lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco (Nicotiana tabacum cv. Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased and their leaf GA1 content was reduced. RNA interference (RNAi) NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and light-saturated capacity of linear electron transport) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue these phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial or detrimental for plant growth. A lycopene β-cyclase expression threshold influences plant biomass, development, and metabolism by modulating pigment and phytohormone content in transplastomic and RNAi tobacco lines. |
| Databáze: | OpenAIRE |
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