Impact of traffic-related air pollution on the expression of Platanus orientalis pollen allergens
| Autor: | Ziba Ghasemi, Maliheh Dadgar Moghadam, Farnaz Sedghy, Abdolreza Varasteh, Mojtaba Sankian, Mahmoud Mahmoudi |
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| Rok vydání: | 2016 |
| Předmět: |
Atmospheric Science
010504 meteorology & atmospheric sciences DNA Plant Health Toxicology and Mutagenesis Air pollution 010501 environmental sciences Biology Iran medicine.disease_cause 01 natural sciences Antibodies Magnoliopsida Pollen Air Pollution Gene expression Botany otorhinolaryngologic diseases medicine Escherichia coli Animals Heavy traffic Cities Air quality index 0105 earth and related environmental sciences Pathogenesis-related protein Plant Proteins Air Pollutants Ecology Platanus orientalis Traffic pollution food and beverages respiratory system Allergens biology.organism_classification Recombinant Proteins Motor Vehicles Microscopy Electron Scanning Female Rabbits |
| Zdroj: | International journal of biometeorology. 61(1) |
| ISSN: | 1432-1254 |
| Popis: | Air pollutants and their interaction with environmental allergens have been considered as an important reason for the recent increase in the prevalence of allergic diseases. The aim of this study was to investigate the traffic pollution effect, as a stressor, on Platanus orientalis pollen allergens messenger RNA (mRNA) and protein expression. P. orientalis pollen grains were collected along main streets of heavy traffic and from unpolluted sites in Mashhad city, in northeast Iran. The pollen samples were examined by scanning electron microscopy. To assess the abundance of pollen allergens (Pla or 1, Pla or 2, and Pla or 3) from polluted and unpolluted sites, immunoblotting was performed. Moreover, the sequences encoding P. orientalis allergens were amplified using real-time PCR. Scanning electron microscopy showed a number of particles of 150–550 nm on the surface of pollen from polluted sites. Also, protein and gene expression levels of Pla or 1 and Pla or 3 were considerably greater in pollen samples from highly polluted areas than in pollen from unpolluted areas (p |
| Databáze: | OpenAIRE |
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