Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome
Autor: | Guozhu Wei, Qiuyue Wang, Jiao Xu, Xiaoyu Ma, Fenqin Chen |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Inflammasomes Glomerular Mesangial Cell Cell Interleukin-1beta Inflammation Pharmacology Protective Agents 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Diabetes mellitus NLR Family Pyrin Domain-Containing 3 Protein medicine Animals MTT assay Diabetic Nephropathies Diabetic kidney disease Naringin Mesangial cell Cell growth Inflammasome General Medicine lcsh:Other systems of medicine NLRP3-caspase-1-IL-1β/IL-18 signaling pathway lcsh:RZ201-999 NLRP3 inflammasome Rats 030104 developmental biology medicine.anatomical_structure Glucose Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Flavanones Mesangial Cells medicine.symptom medicine.drug Research Article |
Zdroj: | BMC Complementary and Alternative Medicine BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-11 (2018) |
ISSN: | 1472-6882 |
Popis: | Background The Nucleotide binding and oligomerization domain-like receptorfamily pyrin domain-containing 3 (NLRP3)-inflammasome plays an important role in various diseases, including a variety of kidney diseases. Naringin exhibits anti-inflammatory and anti-oxidation effects among others, but its specific mechanisms are not clear. We investigated the expression of the NLRP3-inflammasome under high-glucose conditions, assessed the effects of naringin on that process, and further elucidated the role of naringin in the pathogenesis of diabetic kidney disease(DKD). Methods To assess the therapeutic potential of naringin and the mechanisms involved, we cultured rat glomerular mesangial cells and grouped them according to different glucose concentrations, different action times, different concentrations of MCC950, and different concentrations of naringin.The cell proliferation was measured by MTT assay. The expression of Interleukin-1β(IL-1β) and Interleukin18 (IL-18) in the cell supernatant were detected by ELISA. The expression and activity of NLPR3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and Caspase-1 were detected by Western Blot. Results The expressions of NLRP3, ASC, caspase-1, IL-1β, and IL-18 in rat glomerular mesangial cells were significantly higher in the high glucose (HG) group than in the control normal glucose (NG) group and exhibited time-dependence activity. The expression levels of NLRP3, caspase-1, IL-1β, and IL-18 in different treatment groups were significantly lower compared with the HG group after 48 h of MCC950 pre-treatment (p |
Databáze: | OpenAIRE |
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