Measurement of Electron Transport System (ETS) activity in soil

Autor: Colin I. Mayfield, W. E. Inniss, J. T. Trevors
Rok vydání: 2013
Předmět:
Zdroj: Microbial ecology. 8(2)
ISSN: 0095-3628
Popis: Electron transport system (ETS) activity was measured in amended and nonamended soil by measuring the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan), which can be easily extracted with methanol without interference from other compounds found in soil. A high correlation between ETS activity and oxygen consumption was observed. This technique allows rapid quantitative measurements of microbial ETS activity in soil.
Databáze: OpenAIRE