Reduced TNF-α and IFN-γ responses to Central Asian strain 1 and Beijing isolates of Mycobacterium tuberculosis in comparison with H37Rv strain
Autor: | Zahra Hasan, Rabia Hussain, Rumina Hasan, Akbar Kanji, Mahnaz Tanveer |
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Rok vydání: | 2009 |
Předmět: |
Adult
Male Tuberculosis Adolescent Genotype Hydrolases medicine.medical_treatment Mycobacterium tuberculosis Interferon-gamma Young Adult Species Specificity Interferon Prevalence medicine Humans Pakistan Interferon gamma Whole blood biology Tumor Necrosis Factor-alpha Public Health Environmental and Occupational Health General Medicine Middle Aged medicine.disease biology.organism_classification Virology Infectious Diseases Cytokine Female Parasitology Tumor necrosis factor alpha medicine.drug |
Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 103:581-587 |
ISSN: | 0035-9203 |
Popis: | Pakistan ranks eighth in terms of tuberculosis burden worldwide, with an incidence of 181/100000. The predominant genotypes of Mycobacterium tuberculosis are reported to be the Central Asian strain 1 (CAS1) and Beijing families.Mycobacteriumtuberculosis down-regulates host pro-inflammatory cytokines, which are essential for protection against infection. There is currently little information regarding the interaction of the CAS1 genotype with host cells. We studied the growth rates of CAS1 and Beijing clinical isolates, and their ability to induce cytokines compared with the laboratory reference strain H37Rv. Host responses were studied using a THP-1 monocytic cell line model and an ex vivo whole blood assay. Growth rates of CAS1 and Beijing isolates were significantly lower (P=0.011) compared with H37Rv. All clinical isolates induced significantly lower levels of TNF-alpha secretion (P=0.003) than H37Rv in THP-1 cells and in the whole blood assay of healthy donors (n=8). They also induced lower IFN-gamma secretion in the whole blood assay (P0.001). A positive correlation was observed between the growth indices (GI) of H37Rv, Beijing and CAS1 strains and the TNF-alpha responses they induced [Pearson's correlation coefficient (R(2)): 0.936, 0.775 and 0.55, respectively], and also between GI and IFN-gamma production (R(2): 0.422, 0.946, 0.674). These findings suggest that reduced growth rate, together with down-modulation of pro-inflammatory cytokines, is a contributory mechanism for the predominance of the CAS genotype. |
Databáze: | OpenAIRE |
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