Wnt1-induced MAFK expression promotes osteosarcoma cell proliferation
| Autor: | Zhao Zf, Rui Wang, Zhang Ds, Yi Yang, Jie Zheng |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Bone Neoplasms
Wnt1 Protein Biology Transfection Cell Line Tumor Genetics medicine Humans Viability assay RNA Messenger WNT1 Fibrosarcoma Molecular Biology Wnt Signaling Pathway Cell Proliferation Osteosarcoma Oncogene Cell growth Wnt signaling pathway General Medicine Cell cycle medicine.disease MafK Transcription Factor Immunology Cancer research |
| Zdroj: | Genetics and molecular research : GMR. 14(3) |
| ISSN: | 1676-5680 |
| Popis: | Osteosarcoma is one of the most common primary bone tumors in children and young adults. In this study, we investigated the role of musculoaponeurotic fibrosarcoma oncogene homolog K (MAFK) in osteosarcoma cell proliferation in vitro and the possible pathways that contributed to MAFK-related osteosarcoma development. We first reported that MAFK was expressed at low levels in an osteosarcoma cell line. Furthermore, a significant correlation between MAFK and the Wnt signaling pathway was observed in osteosarcoma by using a gene microarray assay. We found that expression of MAFK could be induced by Wnt1 in a dose-dependent manner. Furthermore, Wnt1-induced MAFK expression caused a significant increase of cell viability, whereas a Wnt pathway inhibitor, IWR-1-endo, abolished Wnt1-induced effects on MAFK. Finally, cell cycle analysis showed that enhanced cell proliferation might be attributed to re-distribution of the cell cycle. Together, our results suggested that Wnt1-induced MAFK expression promoted cell proliferation in MG63 cells, and that the role of MAFK in osteosarcoma might be closely linked to the Wnt signaling pathway. |
| Databáze: | OpenAIRE |
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