Intracellular-to-extracellular localization switch of acidic lipase in Enterobacter cloacae through multi-objective medium optimization: aqueous two-phase purification and activity kinetics

Autor: Atim Asitok, Maurice Ekpenyong, Nkpa Ogarekpe, Richard Antigha, Iquo Takon, Anitha Rao, Juliet Iheanacho, Sylvester Antai
Rok vydání: 2022
Předmět:
Zdroj: World Journal of Microbiology and Biotechnology. 38
ISSN: 1573-0972
0959-3993
Popis: As physiological impairments that require replacement therapy continue to increase, so also does the need for improved production of acidic lipase from new microbial sources. Enterobacter cloacae strain UCCM 00116 produced a novel acidic lipase in kernel oil-processing waste-basal broth with 0.023:1 extracellular: intracellular localization ratio. This research re-directed enzyme localization to the extracellular milieu to reduce recovery cost using multi-objective response surface optimization of medium parameters. Results revealed a 1:0.32 extracellular:intracellular lipase ratio. Product formation kinetics, modeled by the Luedeking-Piret function, showed a significant switch from a completely growth-associated intracellular production to a predominantly non-growth-associated extracellular localization through medium optimization. Aqueous two-phase system purification conditions extracted 95.22% lipase with 72.36 purity, a V
Databáze: OpenAIRE
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