Effect of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) on HSP70 and HSP28 gene expression and thermotolerance development in human colon carcinoma cells
| Autor: | Michael J. Borrelli, Geza Erdos, Christine M. Berns, Peter M. Corry, Yong J. Lee, Chang H. Ahn |
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| Rok vydání: | 1994 |
| Předmět: |
Pharmacology
Sulfonamides Hot Temperature Gene Expression Biology Isoquinolines Biochemistry Molecular biology Piperazines Hsp70 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Shock (circulatory) Heat shock protein Gene expression Tumor Cells Cultured medicine Protein biosynthesis Humans HSP70 Heat-Shock Proteins RNA Messenger Northern blot medicine.symptom Protein Kinase C Protein kinase C |
| Zdroj: | Biochemical Pharmacology. 48:2057-2063 |
| ISSN: | 0006-2952 |
| Popis: | The effect of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), a potent protein kinase C (PKC) inhibitor, on the development of thermotolerance and expression of heat shock genes (HSP70 and HSP28) was investigated in human colon carcinoma HT-29 cells. After acute heating at 45 degrees for 15 min, cells became resistant to a challenge heat shock. The development of thermotolerance was suppressed by adding H-7 after heat shock. Northern blots show that the levels of HSP70 and HSP28 mRNA increased rapidly and reached maximal values within 6 hr. H-7 suppressed the accumulation of HSP70 and HSP28 mRNA as well as their protein synthesis, and the level of suppression was concentration dependent. However, little effect was observed if the drug was added 1 hr before and during heat shock. These results suggest that PKC is involved in the regulation of heat shock gene expression after acute heat shock. |
| Databáze: | OpenAIRE |
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