Trypanosoma evansi: Effect of experimental infection on the osmotic fragility, lipid peroxidation and calcium-ATPase activity of rat red blood cells
| Autor: | Ramon Portillo, Alfredo Mijares, Meyerling Betancourt, Jeilyn Vivas, Reinaldo Marín, Sandy Piñero, Fulgencio Proverbio, Cilia Abad |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Trypanosoma medicine.medical_specialty Erythrocytes Time Factors Anemia Immunology Calcium-Transporting ATPases Biology Hematocrit medicine.disease_cause Thiobarbituric Acid Reactive Substances Rats Sprague-Dawley Lipid peroxidation Random Allocation chemistry.chemical_compound Trypanosomiasis hemic and lymphatic diseases Internal medicine medicine Animals Analysis of Variance medicine.diagnostic_test Erythrocyte Membrane Erythrocyte fragility General Medicine Trypanosoma evansi medicine.disease biology.organism_classification Rats Calcium ATPase Osmotic Fragility Red blood cell Infectious Diseases Endocrinology medicine.anatomical_structure chemistry Parasitology Lipid Peroxidation Oxidative stress |
| Zdroj: | Experimental Parasitology. 124:301-305 |
| ISSN: | 0014-4894 |
| Popis: | Trypanosoma evansi is the causative agent of equine trypanosomoses. The disease is characterized by fever, anemia, and cachexia. Peroxidative damage of the red blood cells caused by the parasite, may contribute to the pathogenesis of the anemia seen in trypanosomoses. Consequently, we evaluated the hematocrit, the osmotic fragility of the red blood cells, the level of lipid peroxidation and the activity of the Ca-ATPase of red blood cell ghosts from rats experimentally infected with T. evansi. After 72 h inoculation, the hematocrit decreased from 49.5% to 33%; the osmotic fragility of the red blood cells was approximately 40% higher as compared to the healthy animals; and the red blood cell ghosts showed a higher level of lipid peroxidation and a lower Ca-ATPase activity than the red cell ghosts from the healthy animals. In vitro incubations of red blood cells from healthy animals with T. evansi, produced also a significant increase of the osmotic fragility of the red blood cells. |
| Databáze: | OpenAIRE |
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