Nuclear magnetic resonance study of d-TGGCCA in solution
| Autor: | Jean-Yves Lallemand, Jean Igolen, T. Huyn-Dinh, Eric Guittet, Denis Piveteau |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Coupling constant
Magnetic Resonance Spectroscopy Oligonucleotides Temperature Nuclear magnetic resonance spectroscopy of nucleic acids Nuclear magnetic resonance spectroscopy Nuclear Overhauser effect Biology Molecular physics Dissociation (chemistry) Helix (Snails) Genetics Proton NMR Nucleic Acid Conformation Two-dimensional nuclear magnetic resonance spectroscopy |
| Zdroj: | Nucleic Acids Research. 12:5927-5941 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The 1H NMR spectrum of a self complementary deoxyhexanucleotide (d-TGGCCA) in water solution has been completely and unambiguously assigned by use of two-dimensional techniques and phosphorus off-resonance decoupling experiments. The helix-coil transition has been monitored by following most of the non-exchangeable protons against temperature and was shown to be a cooperative dissociation-association process. Sequential fraying is sizable even at low temperature. Considerations of coupling constants and of Nuclear Overhauser effects show the helix to be of the B-DNA type. |
| Databáze: | OpenAIRE |
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