Unique Aggregation of Sterigmatocystin in Water Yields Strong and Specific Circular Dichroism Response Allowing Highly Sensitive and Selective Monitoring of Bio-Relevant Interactions
| Autor: | Gennaro Pescitelli, Maja Šegvić Klarić, Nataša Šijaković Vujičić, Vilko Smrečki, Marcin Górecki, Ivo Crnolatac, Daniela Jakšić, Ivo Piantanida |
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| Rok vydání: | 2019 |
| Předmět: |
Circular dichroism
Sterigmatocystin Supramolecular chemistry Pharmaceutical Science sterigmatocystin monitoring in water 010501 environmental sciences 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Differential scanning calorimetry non-covalent DNA binding Microscopy Electron Transmission Aspergilli chiral aggregation toxins Drug Discovery lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Calorimetry Differential Scanning Chemistry Circular Dichroism Water DNA Highly sensitive lcsh:Biology (General) Transmission electron microscopy Homogeneous Biophysics Thermodynamics Chirality (chemistry) |
| Zdroj: | Marine Drugs Volume 17 Issue 11 Marine Drugs, Vol 17, Iss 11, p 629 (2019) |
| ISSN: | 1660-3397 |
| Popis: | We demonstrated the hitherto unknown property of the mycotoxin sterigmatocystin (STC) to provide homogeneous solutions in aqueous medium by forming a unique aggregate type (not formed by analogous aflatoxins), characterized by exceptionally strong circular dichroism (CD) bands in the 300&ndash 400 nm range. Results showed that these CD bands do not originate from intrinsic STC chirality but are a specific property of a peculiar aggregation process similar to psi-DNA CD response. Transmission electron microscopy (TEM) experiments revealed a fine fiber network resembling a supramolecular gel structure with helical fibers. Thermodynamic studies of aggregates by differential scanning calorimetry (DSC) revealed high reversibility of the dominant aggregation process. We demonstrated that the novel STC psi-CD band at 345 nm could be applied at biorelevant conditions (100 nanomolar concentration) and even in marine-salt content conditions for specific and quantitative monitoring of STC. Also, we showed that STC strongly non-covalently interacts with ds-DNA with likely toxic effects, thus contrary to the previous belief requiring prior enzyme epoxidation. |
| Databáze: | OpenAIRE |
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