Adhesion of Human Neutrophils to and Activation by Type-I Collagen Involving a β2 Integrin
| Autor: | Jean Dufer, Roselyne Garnotel, Randoux A, J.P. Borel, Jean-Claude Monboisse |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Integrins
Neutrophils Immunology Integrin CD18 Collagen receptor Antigens CD Cell Adhesion Humans Immunology and Allergy Magnesium biology CD11 Antigens Cell Membrane Temperature Antibodies Monoclonal Oxides Cell Biology Adhesion Molecular biology Fibronectins Fibronectin Integrin alpha M Biochemistry CD18 Antigens biology.protein Calcium Integrin beta 6 Collagen Type I collagen |
| Zdroj: | Journal of Leukocyte Biology. 50:373-380 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1002/jlb.50.4.373 |
| Popis: | We previously demonstrated that the α1(l) polypeptide chain of collagen can bind and activate polymorphonuclear neutrophils (PMN). In the present experiments, performed in culture grade 96-well plastic plates coated with collagen, fibronectin, or other proteins, adhesion was assessed by staining the adhering cells after 30 min with crystal violet and measuring absorbance at 560 nm, and activation of PMNs was assessed by measuring the amount of O2- formed. Adhesion occurred at 17 and 37°C but activation at 37°C only. Monoclonal antibody anti-CD 18 inhibited adhesion, showing that the receptor of collagen I on PMNs is a β2 integrin. On the other hand, adhesion of PMNs to fibronectin was inhibited by monoclonal antibodies to CD18 and to CD11b. |
| Databáze: | OpenAIRE |
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