Ultrastructural determination of gingival Langerhans cells in alloxan-induced diabetic rats
| Autor: | Cevat Ayvali, Nesrin Özsoy, Nursel Gül, Hamit Bostanci |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Cytoplasm Langerhans cell Clinical Biochemistry Gingiva Mitochondrion Biology Biochemistry Epithelium Diabetes Mellitus Experimental Pathogenesis Basal (phylogenetics) Microscopy Electron Transmission Diabetes mellitus medicine Animals Rats Wistar Cell Nucleus Endoplasmic reticulum Cell Biology General Medicine medicine.disease Gingivitis Rats medicine.anatomical_structure Langerhans Cells Immunology Ultrastructure |
| Zdroj: | Cell biochemistry and function. 23(3) |
| ISSN: | 0263-6484 |
| Popis: | The ultrastructure of Langerhans cells has not been fully investigated in diabetes-associated gingival tissues. The present study was carried out to investigate the ultrastructure of gingival Langerhans cells in alloxan-induced diabetic rats. Gingival biopsies were obtained from 22 diabetic and 18 control rats. Langerhans cells were observed by transmission electron microscopy (TEM) in the basal layers of healthy oral epithelium. On rare occasions, Langerhans cells were found in the suprabasal layers of the oral epithelium. Langerhans cells in the oral epithelium of diabetic rats were seen in the basal and suprabasal layers. Usually, Langerhans cells had clear cytoplasm and convoluted or indented nuclei and few or no specific granules. The clear cytoplasm contained mitochondria, lysosomes and a small number of rough-surfaced endoplasmic reticulum regions, but it lacked tonofilament. Occasionally, centrioles were also observed in the cytoplasm. The membrane of Langerhans cells had no junctional complexes such as desmosomes. In diabetic rats, Langerhans cell precursors were developed into specific granule-bearing cells. Both Langerhans cells and their granules were more frequent in the gingiva of diabetic rats than in the control group. These data suggest that Langerhans cells play an important role in explaining the pathogenesis and development of diabetic gingivitis. |
| Databáze: | OpenAIRE |
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